axoCells™ SureBond-XF coating, 1 ml

Applications

Our axoCells^TM cortical excitatory neuron progenitors are suitable for investigating Alzheimer’s disease and for screening candidate therapies for this disease.

Healthy control lines are also available and can be used with high–content imaging, MEA, microfluidic and organ-on-chip (OOC) devices to measure structural and functional responses in neuronal models.

Protocols

Fully optimized cell culture reagents are available for the differentiation of progenitors into mature cortical excitatory neurons within 14 days. In addition, single media protocols, enabling co- and tri-culture of our cortical excitatory neurons with inhibitory interneurons, astrocytes and microglia provide convenient and fast culture solutions to better model human diseases in vitro. 

Quality

We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.  

All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind. 

Licenses & Consent

Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into cortical excitatory neurons for commercial use.  

Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg^®, to ensure ethical and biological conformity for your peace of mind. 

Publications

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498259/ 

Garcia-Leon JA, Vitorica J, & Gutierrez A (2019). Use of human pluripotent stem cell-derived cells for neurodegenerative disease modeling and drug screening platform. Future Medicinal Chemistry, 11(11), 1305–1322. doi: 10.4155/fmc-2018-0520 [PubMed] [CrossRef] [Google Scholar] 

Media and Reagents

Using NeurOne cortical neuron supplement, mature cerebral cortical excitatory neurons can be generated in 20 days. We also supply neural plating media,  maintenance media and SureBond –XF coating as standalone items or as a kit. 

Data

ICC images of differentiating striatal neural culture. Cells were fixed and stained on day 10, day 18 and day 33 for markers of striatal neurons (medium spiny neurons): DARPP32, CTIP2, CALBINDIN, GABA and MAP2.

Applications

Our axoCells^TM striatal neuron progenitors are suitable for investigating Huntington’s disease and for screening candidate therapies for this disease.

Healthy control lines are also available and can be used with high–content imaging, MEA, microfluidic and organ-on-chip (OOC) devices to measure structural and functional responses in neuronal models.

Protocols

Fully optimized cell culture reagents are available for the differentiation of progenitors into mature striatal neurons within 31 days. In addition, single media protocols, enabling co- and tri-culture of our striatal neurons with inhibitory interneurons, astrocytes and microglia provide convenient and fast culture solutions to better model human diseases in vitro. 

Quality

We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.  

All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind. 

Licenses & Consent

Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into striatal neurons for commercial use.  

Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg^®, to ensure ethical and biological conformity for your peace of mind. 

Publications

Glaser, T., Shimojo, H., Ribeiro, D.E. et al. ATP and spontaneous calcium oscillations control neural stem cell fate determination in Huntington’s disease: a novel approach for cell clock research. Mol Psychiatry 26, 2633–2650 (2021). https://doi.org/10.1038/s41380-020-0717-5

Mathkar, Pranav P. et al. ‘Characterization of Neurodevelopmental Abnormalities in iPSC-Derived Striatal Cultures from Patients with Huntington’s Disease’. Journal of Huntington’s Disease, vol. 8, no. 3, pp. 257-269, 20191 Jan. 2019 : 257 – 269. DOI: 10.3233/JHD-180333

Media and Reagents

Using our kit of specially formulated striatal neuron media ax0333, growth factors BDNF ax139800 and GDNF ax139855, and SureBond–XF coating, mature striatal neurons can be generated in 31 days. We also supply all reagents as standalone items.