Description
Build high-quality neurodegenerative disease models in 21 days.
Ensure confidence in your neural workflow with high-quality axoCellsTM striatal neurons differentiated from human induced pluripotent stem cells (iPSCs). Derived from consenting donor cord blood and fibroblasts, our striatal neurons have been specifically developed for use in mono-, co- and tri-culture models of neurodegenerative disease and for drug discovery.
The vast majority of human striatal neurons are γ-amino butyric acid (GABAergic) projection medium spiny neurons (MSNs). These cells typically express dopamine and cAMP-regulated phosphoprotein (DARPP32). It is these cells that specifically degenerate in the early phase of Huntington’s disease (HD), causing progressive loss of the patient’s motor and cognitive functions.
Axol has developed a medium formulation that can efficiently drive the differentiation of iPSC-derived neural stem cells to the striatal neuronal lineage. The neurons derived using the medium kit are functionally active and express the key markers of human MSNs (DARPP32, CTIP2, CALBINDIN, and GABA) and form functional synapses in twenty days. With our striatal neurons, you can build robust mono-, co- and tri-culture models with isogenic astrocytes, microglia and cortical inhibitory interneurons to investigate neurological diseases (Alzheimer’s, Parkinson’s, and Huntington’s Disease; epilepsy) and for drug screening and discovery.
Supporting quality and consistency
Confidently carry out your neural workflow with iPSCs manufactured in our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Join our customer base of top ten biopharma and academic institutions in building robust, physiologically relevant disease models for research and drug discovery.
How to get high functioning Striatal Neurons:
- You’ll need a Striatal Neuron Kit ax3115, ax3116, ax3118 or ax3211
- In this you’ll find neural stem cells ax0015, ax0016, ax0018 or ax0211 respectively and all reagents*
- Follow the protocol
- 21 days later you will have high functioning striatal neurons
*Additional third party components may be required. Be sure to check the protocol before you start using the striatal neuron kit.
axoCells Striatal Neuron kits
| Donor gender | Donor age | Source | Condition | Product code | Cells/vials |
| Male | Newborn | Cord blood | Healthy | ax3115 | ≥1.5 million |
| Female | Newborn | Cord blood | Healthy | ax3116 | ≥1.5 million |
| Male | 74 | Pulmonary fibroblast | Healthy | ax3118 | ≥1.5 million |
| Female | 48 | Dermal fibroblast | Huntington’s Disease | ax3211 | ≥1.5 million |
Need a quote for a single product or for the whole cell + media + reagent kit? We’re here to help! Click the ‘get a quote’ button or send an email to operations@axolbio.com
Product Highlights
- Robust, relevant neurons that express key markers of human MSNs such as DARPP32, CTIP2, CALBINDIN and GABA. By using Axol’s striatal neuron medium kit with Huntington’s disease patient-derived NSCs (ax0211), researchers can build their in vitro HD models with the disease relevant cell type (MSNs) and a real patient genetic background (with 50 GAC repeats)
- Ensure confidence with high-quality cells manufactured in our ISO:9001-accredited production facility under rigorous quality control procedures
- Designed for use in mono-, co- and tri-culture models to investigate neurodegenerative diseases and for drug discovery
- Used by leading Biopharma and academic institutions around the world to fuel robust disease models
We have 4 lines from 3 healthy patients and 1 Huntington’s disease patient. For specific info select from the list above or send us a request at operations@axolbio.com
Data
ICC images of differentiating striatal neural culture. Cells were fixed and stained on day 10, day 18 and day 33 for markers of striatal neurons (medium spiny neurons): DARPP32, CTIP2, CALBINDIN, GABA and MAP2.
Applications
Our axoCellsTM striatal neuron progenitors are suitable for investigating Huntington’s disease and for screening candidate therapies for this disease.
Healthy control lines are also available and can be used with high–content imaging, MEA, microfluidic and organ-on-chip (OOC) devices to measure structural and functional responses in neuronal models.
Protocols
Fully optimized cell culture reagents are available for the differentiation of progenitors into mature striatal neurons within 21 days. In addition, single media protocols, enabling co- and tri-culture of our striatal neurons with inhibitory interneurons, astrocytes and microglia provide convenient and fast culture solutions to better model human diseases in vitro.
Quality
We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.
All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into striatal neurons for commercial use.
Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
Glaser, T., Shimojo, H., Ribeiro, D.E. et al. ATP and spontaneous calcium oscillations control neural stem cell fate determination in Huntington’s disease: a novel approach for cell clock research. Mol Psychiatry 26, 2633–2650 (2021). https://doi.org/10.1038/s41380-020-0717-5
Mathkar, Pranav P. et al. ‘Characterization of Neurodevelopmental Abnormalities in iPSC-Derived Striatal Cultures from Patients with Huntington’s Disease’. Journal of Huntington’s Disease, vol. 8, no. 3, pp. 257-269, 20191 Jan. 2019 : 257 – 269. DOI: 10.3233/JHD-180333
Media and Reagents
Using our kit of specially formulated striatal neuron media ax0333, growth factors BDNF ax139800 and GDNF ax139855, and SureBond–XF coating, mature striatal neurons can be generated in 21 days. We also supply all reagents as standalone items.
