Read and download Axol’s validated user protocols for iPSC-derived cells
Change Log
| Protocol | Version/ No. | Section | Change log | Rationale | Previous version | New version |
| Motor Neuron Progenitors | COM/PRT/20 Dec 2023 / version 1 | Preparation of Media and Coating Reagents | Lyophilized PDL changed to liquid PDL and change in concentration used | Enables improved cell adherence and plating consistency | Powdered PDL made up in water and used at 0.1mg/ml | Liquid PDL diluted to 0.05mg/ml |
| Motor Neuron Progenitors | COM/PRT/20 Dec 2023 / version 1 | Coating Cell Culture Ware | Coating incubation time and temperature | Enables improved cell adherence and plating consistency | Incubate plate coated with PDL for 5 mins at room temperature | Incubate plate coated with PDL for 60 mins at 37°C |
| Motor Neuron Progenitors | COM/PRT/20 Dec 2023 / version 1 | Differentiation of axoCells Motor Neuron Progenitors | Seeding density | Optimised cell density based on new coating reagents | 150,000/cm2 | 130,000 – 180,000/cm2 |