Description
Build high-quality in vitro dry AMD models in just 4 weeks.
Ensure confidence in your workflow with high-quality Retinal Pigment Epithelial (RPE) cells differentiated from human induced pluripotent stem cells (iPSCs) and assay-ready in just 4 weeks. Derived from consenting donor PBMCs, our RPE cells have been specifically developed for use in powerful in vitro models and systems for drug discovery.
With Axol, you can rely on robust, physiologically relevant RPE cells that express the key markers (MITF, ZO-1 and PMEL17) and display typical morphology. That means robust, physiologically relevant models of the retina, for ophthalmology research and drug discovery
Supporting quality and consistency
Confidently carry out your workflow with our high-quality iPSCs manufactured with rigorous quality control procedures and guided by decades of scientific experience. All cells come with a full Certificate of Analysis to ensure ethical and biological conformity for your peace of mind.
Join our customer base of biopharma and academic institutions in building robust, physiologically relevant disease models for research and drug discovery.
How to get high-functioning RPE cells:
- You’ll need RPE cells, PCi-RPE_CAU, and all reagents
- Follow the protocol
- 4 weeks later you will have highly functional RPE cells
Cells & kits
| Product name | Cells only code / vial | Quantity* / per vial |
Kit** code |
| axoCells™ human iPSC-derived retinal pigment epithelial cells (unaffected), male donor, aged 30, ≥2 million cells, PCi-RPE | ax8002 | ≥2 million cells | PCi-RPE_KIT |
Media and supplements
| Product name | Product Code | Quantity |
| PhenoCULT® RPE culture media, 100 ml | ax8000 | 100 ml |
| PhenoCULT® RPE culture media, 500 ml | ax8000 | 500 ml |
Need a quote for this single product or for the whole cell + media + reagent kit? We’re here to help! Click the button below or send an email to operations@axolbio.com
Get a quote
Product Highlights
- Derived from human iPSCs
- Assay ready in 4 weeks
- Express the typical markers including MITF, ZO-1 and PMEL17
- Exhibit functional response in phagocytosis assays
- Can be used to fuel advanced in vitro models for toxicology testing, dry AMD research and drug discovery
Phenotypic relevance: Morphology
Human iPSC-derived RPE cells demonstrate typical cobblestone morphology and pigmentation on microscopy.
Phenotypic relevance: ICC
RPE cells express key markers MITF, ZO-1 and PMEL17 on ICC.
Phenotypic relevance: flow cytometry
Flow cytometry demonstrating >95% PMEL 17 expression (blue) against isotype control (black).
Functional relevance: phagocytosis
The phagocytotic potential of iPSC-derived RPE cells was assessed using a pH-Rhodo bioparticle assay (Thermo Fisher), demonstrating phagocytotic activity in 57.2% of the RPE cells, which is within the range described in the literature.
A unique model that recapitulate the main hallmarks of dry AMD
We developed a model that mimic lipofuscin accumulation, a key mechanism in RPE aging and AMD progression
A unique model that recapitulate the main hallmarks of dry AMD
Prolonged A2E treatment followed by blue light illumination recapitulates some of the key pathological mechanisms of AMD: Increased oxidative stress, complement cascade activation, accumulation of drusen-like deposits, and increased expression of inflammatory factors.
Applications
Our RPE cells have been specifically developed for use in advanced in vitro ophthalmology models for toxicology testing, research and drug discovery.
We have a community of biopharma and research institutes that use our RPE cells to build powerful in vitro models for toxicology screening and to model ophthalmic disorders (including dry AMD) for drug discovery.
Protocols
Quality
Guided by over a decade of expertise, we ensure confidence in the quality of our iPSC-derived cells with rigorous QC. All cells come with a full Certificate of Analysis to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into RPE cells for commercial use.
Patient samples used to create these RPE cells have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
TBC
Media and Reagents
- RPE culture medium