Description
Build high-quality neurodegenerative disease models in 7 days.
Ensure confidence in your neural workflow with high-quality axoCellsTM microglia differentiated from human induced pluripotent stem cells (iPSCs). Derived from consenting donor fibroblasts, we’ve specifically designed our production method to equip you with physiologically relevant cells, by mimicking the in vivo pathway of development for brain-resident macrophages.
With Axol, you can rely on physiologically relevant microglia that express the three key markers (IBA1, TMEM119, and P2RY12) and demonstrate robust functional activity, measured by cytokine release, phagocytosis, and chemotaxis. With our microglia, you can build robust mono- and co-culture models to investigate neurological diseases and drug discovery.
Supporting quality and consistency
Confidently carry out your neural workflow with iPSCs manufactured in our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Join our customer base of top ten biopharma and academic institutions in building robust, physiologically relevant disease models for research and drug discovery.
How to get highly functional axoCells Microglia
To ensure simplicity and effectiveness, we’ve developed a kit containing iPSC-derived cells that have been directed down a microglia end-type lineage. Using the easy-to-follow protocol, you can rapidly mature these cells into functional microglia in just 7 days.
- You’ll need a Microglia kit ax0679
- In this you’ll find Microglia cells ax0664 and all reagents*
- Follow the protocol
- 7 days later you will have highly functional microglia
*Additional third party components are required. Be sure to check the protocol before you start using the microglia kit.
Need a quote for this single product or for the whole cell + media + reagent kit? We’re here to help! Click the ‘get a quote’ button or send an email to operations@axolbio.com
Product Highlights
- High-quality, relevant microglia that express the three key markers (IBA–1, TMEM119 and P2RY12) and demonstrate robust functional activity measured by cytokine release, phagocytosis and chemotaxis
- Save time with functionally mature cells available in just 7 days
- Ensure confidence with high-quality cells manufactured in our ISO:9001-accredited production facility under rigorous quality control procedures
- Designed for use in mono-, co- and tri-culture models
- Used by leading Biopharma and academic institutions around the world to fuel robust neurodegenerative disease models and for drug discovery
Applications
Our axoCellsTM microglia have been specifically developed for use in in vitro disease modeling and drug discovery. As inflammation is linked to many neurological diseases such as Alzheimer’s, Parkinson’s, ALS, and aging, microglia are becoming targets for treatments of these conditions.
Axol’s production method mimics the in vivo pathway of development for brain resident macrophages and produces cells that are functionally representative of primary human microglia in vitro. Our microglia have been used extensively in co- and tri-culture with Axol’s cortical excitatory neurons, astrocytes, and/or inhibitory interneurons to produce more relevant models of human diseases such as Alzheimer’s and Parkinson’s.
Protocols
Protocols for incorporating microglia into co- and tri-culture with neurons are available as well as for microglia in monoculture.
Quality
We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.
All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into microglia for commercial use.
Patient samples used to create these cells have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Suzanne Edavettal, et al 2022. Enhanced delivery of antibodies across the blood-brain barrier via TEMs with inherent receptor-mediated phagocytosis, Med, Volume 3, Issue 12, 2022, Pages 860-882.e15, ISSN 2666-6340, https://doi.org/10.1016/j.medj.2022.09.007.
Timothy J. Sargeant, Célia Fourrier 2023. Human monocyte-derived microglia-like cell models: A review of the benefits, limitations and recommendations, Brain, Behavior, and Immunity, Volume 107, 2023, Pages 98-109, ISSN 0889-1591, https://doi.org/10.1016/j.bbi.2022.09.015.
McKee Chloe G., Hoffos Madison, Vecchiarelli Haley A., Tremblay Marie-Ève 2023. Microglia: A pharmacological target for the treatment of age-related cognitive decline and Alzheimer’s disease. Frontiers in Pharmacology 14 2023. http://doi.org10.3389/fphar.2023.1125982
Media & Reagents
A ready to go kit containing microglia cells and optimized media is available Microglia Complete Cells and Media (ax0679) or media is available as a standalone item Microglia maintenance media kit (ax0660).
