axoServices^TM iPSC Differentiation

Outsource your iPSC project to the experts

Outsource your iPSC differentiation project to the experts and prioritize quality, experience and confidence

Make the right choice, first time

With over a decade of experience in the human iPSC space, we understand that high-quality differentiation is challenging, with significant risks and costs if not performed well. But like you, we also see the enormous value of human iPSCs for building better, more physiologically relevant models of human disease.

That’s why we offer our iPSC expertise to leading biopharma and research organizations globally. We’ve developed a tried-and-tested workflow to provide full-service differentiation from iPSCs to end-point cells, with robust characterization and ongoing support from our expert scientists.

Clients who work with Axol share our obsession with doing it once and doing it well. We prioritize quality control and characterization, always built-in as standard in our projects, to ensure there are no surprise challenges (or costs), maximizing project success. That gives you more confidence, time and resource to focus on your research to produce better human disease models for drug discovery and beyond.

Choose an axoServices^TM Differentiation project for:

Prioritization of quality and consistency

Open discussions with actual iPSC experts

Dependable, high-quality project outcomes

“Working with AXOL has been a professional experience all around. Data was delivered on time and presented in a clear and concise fashion. Our expectations were met, and we plan to work together again.”

Dr Ian Moench, GSK

What do you get with Axol?

Step 1. Kick-off meeting with the Axol iPSC experts

Decades of experience in the room
Open, two-way communication
Rapid follow-up with competitive quote

Step 2. Extensive iPSC characterization

Flow cytometry to confirm pluripotency-associated marker expression
Ensure high-quality starting material to maximize project success

Step 3. Directed differentiation + optimization

Our proprietary directed differentiation process uses small molecules to generate footprint-free end-point cells
Built-in optimization provides safety net for project challenges
Robust target cell populations for QC, custom assays and shipping

Step 4. QC and custom assays

Rigorous checks for contaminants and optimal post-thaw viability
ICC to confirm expression lineage-specific markers
Custom assays capabilities to match project requirements
Desired end-point cells are shipped with media and instructions

Step 5. Ongoing support

Continued communication and advice from iPSC experts
Ease the adaptation of cells into your experimental system
An open channel for future projects, collaborations and partnerships

Example cases

Brief: A client would like to produce microglia for an Alzheimer’s monoculture disease model, using cells from Axol’s banks.

Project: Axol iPSC line ax0113 (derived from an Alzheimer’s patient) was already well-characterized for both cell culture performance and physiological, end-point performance. As a result, differentiation using our proprietary small molecule process went smoothly.

Outcome: Generation of 30 vials of microglia within 6 weeks (plus extensive QC). Additional custom assays (chemotaxis and phagocytosis) were performed and reported.

Brief: A client would like us to differentiate their cells into sensory neurons for use in monoculture, before consideration of co-culture.

Project: Initial characterization revealed significant quality issues with the source material. However, Axol provided advice and additional optimization steps to account for the challenges, communicating this with the client.

Outcome: Generation of 30 vials of sensory neurons in 8 weeks, plus extensive QC. By identifying and communicating challenges up-front, there was full transparency with the additional time and resources for project completion.

We offer differentiation of iPSCs to the following cell types:

Neurons and neuroinflammatory cells

Cortical inhibitory interneurons
Cortical excitatory neurons
Striatal neurons
Astrocytes
Microglia
Sensory neurons
Motor neurons

Cardiac cells

Ventricular cardiomyocytes
Atrial cardiomyocytes

Our approach to collaboration

What does the ideal outsourced scientific project look like? We see ourselves as collaborators, partners, bench scientists and project managers, here to work alongside you to support and accelerate your work. And just like you, we like getting the work done efficiently and effectively, with clear open communication throughout.

We also recognise the trust you put in us to support your work, remove the heavy lifting and ensure your timelines proceed on schedule. Ultimately, we’re all progressing towards the same mission so we would be honoured to partner on your research and get closer to delivering better therapies for patients.

Our company operates through its stated values and behaviours of Performance, Accountability, Transparency, Collaboration, Diversity & Inclusion and Integrity. With a dedicated services team, we’re always on hand to support in any way we can.

We hope that we can be of service and welcome your enquiries.

Case Study