Description
Produce mature sebocytes in 4 days.
Ensure confidence in your workflow with high-quality Sebocytes differentiated from human induced pluripotent stem cells (iPSCs). Derived from consenting donor PBMCs, our sebocytes have been specifically developed for use in powerful in vitro monoculture models, and complex co-culture systems for drug discovery and cosmetics research.
Rely on robust, physiologically relevant sebocytes that express the key markers (MUC1 and KRT7) and display typical morphology. That means robust, physiologically relevant models of skin for drug discovery, toxicology, cosmetics, and dermatology research.
Supporting quality and consistency
Confidently carry out your workflow with our high-quality iPSCs manufactured with rigorous quality control procedures and guided by decades of scientific experience. All cells come with a full Certificate of Analysis to ensure ethical and biological conformity for your peace of mind.
Join our customer base of top pharma and cosmetics companies in building robust, physiologically relevant models for research and drug discovery
How to get high-functioning sebocytes:
- You’ll need Sebocytes PCi-SEB_CAU, and all reagents
- Follow the protocol
- 4 days later you will have highly functional sebocytes
Cells & kits
| Product name | Cells only code / vial | Quantity* / per vial |
Kit** code |
| PCi-SEB Human iPSC-Derived Sebocytes | PCi-SEB_CAU | ≥2 million cells | PCi-SEB_CAU_KIT |
Media and Supplements
| Product name | Product Code | Quantity |
| Sebocyte Culture Media (100 ml) | PhenoCULT®-SEB_100 | 100 ml |
| Sebocyte Culture Media (500 ml) | PhenoCULT®-SEB_500 | 500 ml |
Need a quote for this single product or for the whole cell + media + reagent kit? We’re here to help! Click the button below or send an email to operations@axolbio.com
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Product Highlights
- Derived from human iPSCs
- Assay ready in 4 days
- Express the typical markers MUC1 and KRT7
- Produce sebum and exhibit functional responses to testosterone and linoleic acid
- Used by leading biopharma and cosmetics companies to fuel advanced in vitro models for cosmetics research, toxicology and drug discovery
Phenotypic relevance: Morphology
PCI-SEB human iPSC-derived sebocytes exhibit typical epithelial morphology of primary sebocytes with heterogeneity in cell size due to lipid accumulation. Magnification
Phenotypic relevance: ICC
PCI-SEB sebocytes express the key markers MUC1 (MUCIN 1) and KRT7 (KERATIN 7).
Phenotypic relevance: flow cytometry
PCI-SEB sebocytes exhibit >90% purity as measured by KRT7+ expression. Black: isotype control ; Red: anti-KRT7 antibody.
Phenotypic relevance: RT-qPCR
Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 days.
Phenotypic relevance: RT-qPCR
Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 day.
Functional relevance: response to testosterone
PCI-SEB sebocytes respond to a 96h treatment with testosterone (10 µM) by a 2-fold increase in lipid content. This response is significantly inhibited by the 5-α-reductase inhibitor finasteride (10 µM).
Applications
PCi-SEB Sebocytes are unique tools for dermatology research, pharmacology, and drug discovery, including 3D skin reconstruction
We have a community of biopharma and cosmetics companies that use our sebocytes to build powerful in vitro models to investigate cosmetic toxicology and to model skin disorders (including acne) for drug discovery.
Quality
Guided by over a decade of expertise, we ensure confidence in the quality of our iPSC-derived cells with rigorous QC. All cells come with a Cellular Analysis Report to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into sebocytes for commercial use.
Patient samples used to create these sebocytes have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
Makrantonaki, E., Ganceviciene, R., & Zouboulis, C. (2011). An update on the role of the sebaceous gland in the pathogenesis of acne. Dermatoendocrinol, 3(1), 41-49. doi:10.4161/derm.3.1.13900
Pappas, A., Fantasia, J., & Chen, T. (2013). Age and ethnic variations in sebaceous lipids. Dermatoendocrinol, 5(2), 319-324. doi:10.4161/derm.2536
Xia, L., Zouboulis, C. C., & Ju, Q. (2009). Culture of human sebocytes in vitro. Dermatoendocrinol, 1(2), 92-95.
Media and Reagents
- Sebocyte culture medium