In partnership with patient advocacy groups and industrial collaborators, we have pledged to help democratize access to physiologically relevant human models of neurodegenerative disease. The Early Access axoCells™ derived from patient-derived iPSCs are widely available to the global scientific community.
axoCells™ human iPSC-derived cortical inhibitory neurons (unaffected), male donor, aged 40-50 ≥1 million cells
Features
- Derived from human iPSCs
- Assay ready in just 20 days
- Express the key markers including GAD65, Parvalbumin, GABA and Somatostatin
- Demonstrated functionality in advanced in vitro models, including co-culture and tri-culture
- RNAseq and TempO-Seq™ data available on request
Specifications
Number of cells: ≥1 million cells
Donor: male, 40-50-year-old
Genetics: healthy control
Price:
$968.00
Description
axoCells human iPSC-derived inhibitory interneurons (≥1 million cells) for maturation to inhibitory interneurons for neuroscience research. Inhibitory interneurons made from iPSCs generated from a 40-50-year-old male donor’s fibroblasts.
Key highlights include:
• Assay ready in just 20 days
• Express the key markers including GAD65, Parvalbumin, GABA and Somatostatin
• Demonstrated functionality in advanced in vitro models including co-culture and tri-culture
These cells have been used in co-culture methodologies to study Alzheimer’s disease, seizure and epilepsy
User Guides
Our User Guides provide detailed protocols, however we recommend using standard optimization techniques and monitoring cells carefully to take into account the intrinsic differences in iPSC-derived cells.
If you would like to discuss the protocols, disease models, or suitable endpoint assays in more detail, please reach out to our scientific team.
Applications
Our axoCellsTM cortical inhibitory interneurons can be used in co-culture with axoCellsTM cortical excitatory neurons to investigate the connections in the brain and also monitor the effect of inhibitory neurons on the electrical activity of these excitatory neurons, mimicking conditions such as epilepsy. In order to create more physiologically relevant models, the inclusion of microglia and/or astrocytes is also possible.
Quality
As an Early Access Product, these cells will not have been manufactured at our ISO accredited facility and final protocols, documentation and literature may still be development.
We welcome feedback on these products from the scientific community. These products are available to purchase at a discounted package price and will be supplied with a feedback form.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into cortical inhibitory interneurons for commercial use.
Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. These early access cells come with a cellular analysis datasheet and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
Cruz-Santos M, Cardo LF, Li M. A Novel LHX6 Reporter Cell Line for Tracking Human iPSC-Derived Cortical Interneurons. Cells. 2022 Mar 1;11(5):853. doi: 10.3390/cells11050853.
PMID: 35269475; PMCID: PMC8909769.
Dubonyte, U., Asenjo-Martinez, A., Werge, T. et al. Current advancements of modelling schizophrenia using patient-derived induced pluripotent stem cells. acta neuropathol commun 10, 183 (2022). https://doi.org/10.1186/s40478-022-01460-2
Ni, P., Noh, H., Park, GH. et al. iPSC-derived homogeneous populations of developing schizophrenia cortical interneurons have compromised mitochondrial function. Mol Psychiatry 25, 2873–2888 (2020). https://doi.org/10.1038/s41380-019-0423-3
Media & Reagents
We supply optimized media and reagents for axoCells cortical inhibitory interneuron culture, whether alone or in co- or tri-culture with other neurons or glia cells. Neural Maintenance Media (ax0031), NeurOne Cortical Neuron Supplement (ax0679), Neural Plating Media (ax0033) and SureBond-XF (ax0053) coating are all available as standalone products or the culture media and coating are available in a kit (Neural Stem Cell Media & Reagent Bundle ax0105) containing ax0674, ax0031 and ax0053.
