Description
Build high-quality neurodegenerative disease models in 20 days.
Ensure confidence in your workflow with high-quality axoCellsTM cortical inhibitory interneurons, differentiated from human induced pluripotent stem cells (iPSCs). Derived from two different consenting donors (newborn male and 40-50yr old male), our inhibitory interneurons have been specifically developed for use in mono-, co- and tri-culture models to investigate neurodegenerative diseases and for drug discovery.
Our robust, physiologically relevant neurons have been shown to express the key cell markers (parvalbumin, somatostatin, tyrosine hydroxylase, PAX-6, MAP2, beta-tubulin, GABA receptor, and GAD enzyme). And with a maturity time of just twenty days, you can build robust models in less time.
Early Access Offer package
Our inhibitory interneurons are available through our Early Access Program where we are offering the following options:
- When you purchase a co-culture or tri-culture kit, you will receive 1 x a0662 and 1 x ax0667 interneurons free of charge.
- If you wish to buy interneurons on their own, you can request a 50% discount on the purchase of a twin pack of 1 x ax0662 and 1 x ax0667.
Need a quote for this single product or for the whole cell + media + reagent kit? We’re here to help! Click the ‘get a quote’ button or send an email to operations@axolbio.com
Product Highlights
- Robust, relevant interneurons express eight key markers including parvalbumin, PAX-6 and MAP2
- Produce mature cells in just 20 days, with functional calcium responses in 30 days
- Specifically designed for use in mono-, co- and tri-culture models to investigate neurodegenerative disorders and for drug discovery
- Used by leading Biopharma and academic institutions around the world to fuel robust mono, co- and tri-culture models
Applications
Our axoCellsTM cortical inhibitory interneurons can be used in co-culture with axoCellsTM cortical excitatory neurons to investigate the connections in the brain and also monitor the effect of inhibitory neurons on the electrical activity of these excitatory neurons, mimicking conditions such as epilepsy. In order to create more physiologically relevant models, the inclusion of microglia and/or astrocytes is also possible.
Protocols
Mono- and co-culture protocols for interneurons alone or with cortical excitatory neurons are available. In addition, we have protocols for tri-culture with astrocytes or microglia using one media for all cell types.
Quality
As an Early Access Product, these cells will not have been manufactured at our ISO accredited facility and final protocols, documentation and literature may still be development.
We welcome feedback on these products from the scientific community. These products are available to purchase at a discounted package price and will be supplied with a feedback form.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into cortical inhibitory interneurons for commercial use.
Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. These early access cells come with a cellular analysis datasheet and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
Cruz-Santos M, Cardo LF, Li M. A Novel LHX6 Reporter Cell Line for Tracking Human iPSC-Derived Cortical Interneurons. Cells. 2022 Mar 1;11(5):853. doi: 10.3390/cells11050853.
PMID: 35269475; PMCID: PMC8909769.
Dubonyte, U., Asenjo-Martinez, A., Werge, T. et al. Current advancements of modelling schizophrenia using patient-derived induced pluripotent stem cells. acta neuropathol commun 10, 183 (2022). https://doi.org/10.1186/s40478-022-01460-2
Ni, P., Noh, H., Park, GH. et al. iPSC-derived homogeneous populations of developing schizophrenia cortical interneurons have compromised mitochondrial function. Mol Psychiatry 25, 2873–2888 (2020). https://doi.org/10.1038/s41380-019-0423-3
Media & Reagents
We supply optimized media and reagents for axoCells cortical inhibitory interneuron culture, whether alone or in co- or tri-culture with other neurons or glia cells. Neural Maintenance Media (ax0031), NeurOne Cortical Neuron Supplement (ax0679), Neural Plating Media (ax0033) and SureBond-XF (ax0053) coating are all available as standalone products or the culture media and coating are available in a kit (Neural Stem Cell Media & Reagent Bundle ax0105) containing ax0674, ax0031 and ax0053.
