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axoCells™ cortical neuron media, supplement and coating kit
axoCells Neural Maintenance Media (500ml), NeurOne supplements A (1 ml) and B (1 ml) and SureBond-XF coating (1 ml), optimized for maturation of cortical neurons for neuroscience research. Suitable for use with axoCells Cortical Neurons and Inhibitory Interneurons.
• Optimized for maturation of cortical neurons for neuroscience research
• Compiled into a convenient bundle format
Specifications
Volume 500 ml + 1ml + 1ml + 1ml
Shipping conditions Dry ice
Storage conditions -80°C
Description
axoCells Neural Maintenance Media (500ml), NeurOne supplements A (1 ml) and B (1 ml) and SureBond-XF coating (1 ml), optimized for maturation of cortical neurons for neuroscience research. Suitable for use with axoCells Cortical Neurons and Inhibitory Interneurons.
The neurOne supplement is engineered to work alongside axoCells Neural Stem Cells (NSCs; ax0015, ax0016, ax0018, ax0111, ax0112, ax0113, ax0114, ax0211, ax0411), to derive cortical excitatory neurons which are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions.
Within 21 days the cortical neurons display typical morphology and express such markers as TBR1 Beta3 Tubulin throughout. The Neur0ne supplement is designed to derive pure cortical neuronal cultures, hence the cultures are also show negative expression of GFAP (Glial Marker) , KI67 (Cell proliferation), Sox 2 (Pluripotency) and Pax 6
The neurOne supplement comes conveniently frozen as a 1ml aliquot for supplement A and B which is sufficient to treat a 96-well plate for the required time.
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Additional Information
Applications
Our axoCellsTM cortical excitatory neuron progenitors are suitable for investigating Alzheimer’s disease and for screening candidate therapies for this disease.
Healthy control lines are also available and can be used with high–content imaging, MEA, microfluidic and organ-on-chip (OOC) devices to measure structural and functional responses in neuronal models.
Protocols
Fully optimized cell culture reagents are available for the differentiation of progenitors into mature cortical excitatory neurons within 14 days. In addition, single media protocols, enabling co- and tri-culture of our cortical excitatory neurons with inhibitory interneurons, astrocytes and microglia provide convenient and fast culture solutions to better model human diseases in vitro.
Quality
We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.
All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into cortical excitatory neurons for commercial use.
Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498259/
Garcia-Leon JA, Vitorica J, & Gutierrez A (2019). Use of human pluripotent stem cell-derived cells for neurodegenerative disease modeling and drug screening platform. Future Medicinal Chemistry, 11(11), 1305–1322. doi: 10.4155/fmc-2018-0520 [PubMed] [CrossRef] [Google Scholar]
Media and Reagents
Using NeurOne cortical neuron supplement, mature cerebral cortical excitatory neurons can be generated in 20 days. We also supply neural plating media, maintenance media and SureBond –XF coating as standalone items or as a kit.
