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axoCells™ neural maintenance-XF media, 500 ml
axoCells™ neural maintenance-XF media, 500 ml is animal-free and optimized for maximum post-thaw recovery of neural cells for neuroscience research. Suitable for use with axoCells Cortical Neurons and Inhibitory Interneurons.
•Xeno-free cell culture medium for optimal maintenance of human neural stem cells and neural cell types.
•Provides an ideal environment to support axonal elongation and synaptic activity.
•Fully-defined medium ensures lot-to-lot consistency and reliable performance between experiments
Specifications
Volume 500ml
Shipping conditions Dry ice
Storage conditions -80°C
Description
axoCells™ neural maintenance-XF media, 500 ml is a xeno-free cell culture medium for optimal maintenance of human neural stem cells and neural cell types.
For long-term neuronal cultures, axoCells Neural Maintenance-XF Media provides an ideal environment to support axonal elongation and synaptic activity. Neural stem cells cultured in this medium maintain the capacity to differentiate into neuronal and glial lineages. The medium is completely defined ensuring lot-to-lot consistency and reliable performance between experiments. Neural Maintenance-XF Medium can maintain healthy neurons when cultured at low density (10,000 cells/cm2). It is available as a standalone product.
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Additional Information
Cortical Excitatory Neurons
Applications
Our axoCellsTM cortical excitatory neuron progenitors are suitable for investigating Alzheimer’s disease and for screening candidate therapies for this disease.
Healthy control lines are also available and can be used with high–content imaging, MEA, microfluidic and organ-on-chip (OOC) devices to measure structural and functional responses in neuronal models.
Protocols
Fully optimized cell culture reagents are available for the differentiation of progenitors into mature cortical excitatory neurons within 14 days. In addition, single media protocols, enabling co- and tri-culture of our cortical excitatory neurons with inhibitory interneurons, astrocytes and microglia provide convenient and fast culture solutions to better model human diseases in vitro.
Quality
We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.
All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into cortical excitatory neurons for commercial use.
Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498259/
Garcia-Leon JA, Vitorica J, & Gutierrez A (2019). Use of human pluripotent stem cell-derived cells for neurodegenerative disease modeling and drug screening platform. Future Medicinal Chemistry, 11(11), 1305–1322. doi: 10.4155/fmc-2018-0520 [PubMed] [CrossRef] [Google Scholar]
Media and Reagents
Using NeurOne cortical neuron supplement, mature cerebral cortical excitatory neurons can be generated in 20 days. We also supply neural plating media, maintenance media and SureBond –XF coating as standalone items or as a kit.
Cortical Inhibitory Interneurons
Applications
Our axoCellsTM cortical inhibitory interneurons can be used in co-culture with axoCellsTM cortical excitatory neurons to investigate the connections in the brain and also monitor the effect of inhibitory neurons on the electrical activity of these excitatory neurons, mimicking conditions such as epilepsy. In order to create more physiologically relevant models, the inclusion of microglia and/or astrocytes is also possible.
Protocols
Mono- and co-culture protocols for interneurons alone or with cortical excitatory neurons are available. In addition, we have protocols for tri-culture with astrocytes or microglia using one media for all cell types.
Quality
As an Early Access Product, these cells will not have been manufactured at our ISO accredited facility and final protocols, documentation and literature may still be development.
We welcome feedback on these products from the scientific community. These products are available to purchase at a discounted package price and will be supplied with a feedback form.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into cortical inhibitory interneurons for commercial use.
Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. These early access cells come with a cellular analysis datasheet and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
Cruz-Santos M, Cardo LF, Li M. A Novel LHX6 Reporter Cell Line for Tracking Human iPSC-Derived Cortical Interneurons. Cells. 2022 Mar 1;11(5):853. doi: 10.3390/cells11050853.
PMID: 35269475; PMCID: PMC8909769.
Dubonyte, U., Asenjo-Martinez, A., Werge, T. et al. Current advancements of modelling schizophrenia using patient-derived induced pluripotent stem cells. acta neuropathol commun 10, 183 (2022). https://doi.org/10.1186/s40478-022-01460-2
Ni, P., Noh, H., Park, GH. et al. iPSC-derived homogeneous populations of developing schizophrenia cortical interneurons have compromised mitochondrial function. Mol Psychiatry 25, 2873–2888 (2020). https://doi.org/10.1038/s41380-019-0423-3
Media & Reagents
We supply optimized media and reagents for axoCells cortical inhibitory interneuron culture, whether alone or in co- or tri-culture with other neurons or glia cells. Neural Maintenance Media (ax0031), NeurOne Cortical Neuron Supplement (ax0679), Neural Plating Media (ax0033) and SureBond-XF (ax0053) coating are all available as standalone products or the culture media and coating are available in a kit (Neural Stem Cell Media & Reagent Bundle ax0105) containing ax0674, ax0031 and ax0053.
