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axoCells™ human iPSC-derived striatal neurons (ax0015, unaffected), media, supplements and coating kit, ≥1.5 million cells
axoCells human iPSC-derived neural stem cells (≥1.5 million cells), striatal neuron basal media (2 x 250ml), striatal neuron media supplement (2 x 7.5ml), differentiation supplement A (1 vial), GDNF (10 µg), BDNF (10 µg), and SureBond-XF coating (1 ml), optimized for maturation of striatal neurons for neuroscience research. NSCs made from iPSCs generated from a newborn male donor’s cord blood.
• Derived from human iPSCs
• Convenient kit format containing cells, media, supplements and coating
• Assay-ready in 31 days
• Express the key markers including DARPP32, CTIP2, CALBINDIN, and GABA
• Demonstrated functionality in advanced in vitro models including co-culture and tri-culture
Kit components
- Human iPSC-derived neural stem cells (ax0015)
- Striatal neuron medium kit
- Recombinant human brain-derived neurotrophic factor (BDNF)
- Recombinant human glial-derived neurotrophic factor (GDNF)
- SureBond-XF
Specifications
Number of cells ≥1.5 million cells
Donor Male, newborn
Genetics Healthy control
Description
axoCells human iPSC-derived neural stem cells (≥1.5 million cells), striatal neuron basal media (2 x 250ml), striatal neuron media supplement (2 x 7.5ml), differentiation supplement A (1 vial), GDNF (10 µg), BDNF (10 µg), and SureBond-XF coating (1 ml), optimized for maturation of striatal neurons for neuroscience research. NSCs made from iPSCs generated from a newborn male donor’s cord blood.
Key highlights include:
• Assay-ready in 31 days
• Express the key markers including DARPP32, CTIP2, CALBINDIN, and GABA
• Demonstrated functionality in advanced in vitro models including co-culture and tri-culture
Top Resources
Additional Information
Striatal Neurons
Data
ICC images of differentiating striatal neural culture. Cells were fixed and stained on day 10, day 18 and day 33 for markers of striatal neurons (medium spiny neurons): DARPP32, CTIP2, CALBINDIN, GABA and MAP2.
Applications
Our axoCellsTM striatal neuron progenitors are suitable for investigating Huntington’s disease and for screening candidate therapies for this disease.
Healthy control lines are also available and can be used with high–content imaging, MEA, microfluidic and organ-on-chip (OOC) devices to measure structural and functional responses in neuronal models.
Protocols
Fully optimized cell culture reagents are available for the differentiation of progenitors into mature striatal neurons within 31 days. In addition, single media protocols, enabling co- and tri-culture of our striatal neurons with inhibitory interneurons, astrocytes and microglia provide convenient and fast culture solutions to better model human diseases in vitro.
Quality
We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.
All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into striatal neurons for commercial use.
Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
Glaser, T., Shimojo, H., Ribeiro, D.E. et al. ATP and spontaneous calcium oscillations control neural stem cell fate determination in Huntington’s disease: a novel approach for cell clock research. Mol Psychiatry 26, 2633–2650 (2021). https://doi.org/10.1038/s41380-020-0717-5
Mathkar, Pranav P. et al. ‘Characterization of Neurodevelopmental Abnormalities in iPSC-Derived Striatal Cultures from Patients with Huntington’s Disease’. Journal of Huntington’s Disease, vol. 8, no. 3, pp. 257-269, 20191 Jan. 2019 : 257 – 269. DOI: 10.3233/JHD-180333
Media and Reagents
Using our kit of specially formulated striatal neuron media ax0333, growth factors BDNF ax139800 and GDNF ax139855, and SureBond–XF coating, mature striatal neurons can be generated in 31 days. We also supply all reagents as standalone items.
