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axoCells™ human iPSC-derived sebocytes (ax6616, unaffected), culture media and supplement kit, PCi-SEB_CAU_KIT
Human iPSC-Derived Sebocytes (≥ 2 million cells), Sebocyte Culture Media (100ml) and Supplement A (100 µl) for cosmetics research. Sebocytes made from iPSCs generated from a 30 year old Caucasian male donor’s PBMCs.
• Derived from human iPSCs
• Assay ready in 4 days
• Convenient kit format containing cells, media and supplements
• Express the typical markers including MUC1 and KRT7
• Exhibit functional response to testosterone and linoleic acid
• Can be used to fuel advanced in vitro models for cosmetics research, toxicology and drug discovery
Kit components
The PCi-SEB_KIT contains:
- 1 vial of PCi-SEB sebocytes (2 M cells/0.5 mL)
- PhenoCULT®-SEB culture medium (100 mL)
- Supplements: 1 vial of Supplement A (100 µL)
*Supplement A (Amplification) is used for amplification of PCi-SEB, keeping a low lipid content.
Specifications
Number of cells ≥2 million
Donor Male, 30 years old
Genetics Healthy control
Description
Human iPSC-Derived Sebocytes Kit containing Sebocytes (≥2 million cells), Sebocyte Culture Media (100ml) and Supplement A (100 µl) for cosmetics research. Sebocytes made from iPSCs generated from a 30 year old Caucasian male donor’s PBMCs.
Key highlights include:
• Derived from human iPSCs
• Assay ready in 4 days
• Convenient kit format containing cells, media and supplements
• Express the typical markers including MUC1 and KRT7
• Exhibit functional response to testosterone and linoleic acid
• Can be used to fuel advanced in vitro models for cosmetic research, toxicology and drug discovery
These cells are frequently used in in vitro skin models, including acne models, for dermatology research and drug discovery.
Top Resources
Additional Information
Phenotypic relevance: Morphology
PCI-SEB human iPSC-derived sebocytes exhibit typical epithelial morphology of primary sebocytes with heterogeneity in cell size due to lipid accumulation. Magnification
Phenotypic relevance: ICC
PCI-SEB sebocytes express the key markers MUC1 (MUCIN 1) and KRT7 (KERATIN 7).
Phenotypic relevance: flow cytometry
PCI-SEB sebocytes exhibit >90% purity as measured by KRT7+ expression. Black: isotype control ; Red: anti-KRT7 antibody.
Phenotypic relevance: RT-qPCR
Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 day.
Phenotypic relevance: RT-qPCR
Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 day.
Functional relevance: response to testosterone
PCI-SEB sebocytes respond to a 96h treatment with testosterone (10 µM) by a 2-fold increase in lipid content. This response is significantly inhibited by the 5-α-reductase inhibitor finasteride (10 µM).
Applications
PCi-SEB Sebocytes have been specifically developed for use in advanced in vitro models of skin for cosmetic toxicology testing, research and drug discovery.
We have a community of biopharma and cosmetics companies that use our sebocytes to build powerful in vitro models to investigate cosmetic toxicology and to model skin disorders (including acne) for drug discovery.
Quality
Guided by over a decade of expertise, we ensure confidence in the quality of our iPSC-derived cells with rigorous QC. All cells come with a full Certificate of Analysis to ensure ethical and biological conformity for your peace of mind.
Publications
Makrantonaki, E., Ganceviciene, R., & Zouboulis, C. (2011). An update on the role of the sebaceous gland in the pathogenesis of acne. Dermatoendocrinol, 3(1), 41-49. doi:10.4161/derm.3.1.13900
Pappas, A., Fantasia, J., & Chen, T. (2013). Age and ethnic variations in sebaceous lipids. Dermatoendocrinol, 5(2), 319-324. doi:10.4161/derm.2536
Xia, L., Zouboulis, C. C., & Ju, Q. (2009). Culture of human sebocytes in vitro. Dermatoendocrinol, 1(2), 92-95.
Media and Reagents
- Sebocyte culture medium
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into sebocytes for commercial use.
Patient samples used to create these sebocytes have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.