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PhenoCULT® sebocyte culture media, 500 ml
PhenoCULT®-SEB : Culture medium for human sebocytes. Basal Media (500ml), and Supplement A (5 x 100µl). Suitable for use with PCi-SEB Sebocytes.
- Optimized for the maturation of sebocytes
- Tested for bacterial, fungal and mycoplasma contamination
Specifications
Volume 500ml
Storage conditions store at – 20◦ C upon receipt.
Description
PhenoCULT®-SEB : Culture medium for human sebocytes. Basal Media (500mL), and Supplement A (5 x 100µl). Suitable for use with PCi-SEB Sebocytes.
Note: Supplement M (for accelerating the maturation of PCi-SEB) is not part of the kit
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Additional Information
Phenotypic relevance: Morphology
PCI-SEB human iPSC-derived sebocytes exhibit typical epithelial morphology of primary sebocytes with heterogeneity in cell size due to lipid accumulation. Magnification
Phenotypic relevance: ICC
PCI-SEB sebocytes express the key markers MUC1 (MUCIN 1) and KRT7 (KERATIN 7).
Phenotypic relevance: flow cytometry
PCI-SEB sebocytes exhibit >90% purity as measured by KRT7+ expression. Black: isotype control ; Red: anti-KRT7 antibody.
Phenotypic relevance: RT-qPCR
Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 day.
Phenotypic relevance: RT-qPCR
Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 day.
Functional relevance: response to testosterone
PCI-SEB sebocytes respond to a 96h treatment with testosterone (10 µM) by a 2-fold increase in lipid content. This response is significantly inhibited by the 5-α-reductase inhibitor finasteride (10 µM).
Applications
PCi-SEB Sebocytes have been specifically developed for use in advanced in vitro models of skin for cosmetic toxicology testing, research and drug discovery.
We have a community of pharma and cosmetics companies that use our sebocytes to build powerful in vitro models to investigate cosmetic toxicology and to model skin disorders (including acne) for drug discovery.
Quality
Guided by over a decade of expertise, we ensure confidence in the quality of our iPSC-derived cells with rigorous QC. All cells come with a full Certificate of Analysis to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
TBC
Publications
Makrantonaki, E., Ganceviciene, R., & Zouboulis, C. (2011). An update on the role of the sebaceous gland in the pathogenesis of acne. Dermatoendocrinol, 3(1), 41-49. doi:10.4161/derm.3.1.13900
Pappas, A., Fantasia, J., & Chen, T. (2013). Age and ethnic variations in sebaceous lipids. Dermatoendocrinol, 5(2), 319-324. doi:10.4161/derm.2536
Xia, L., Zouboulis, C. C., & Ju, Q. (2009). Culture of human sebocytes in vitro. Dermatoendocrinol, 1(2), 92-95.
Media and Reagents
- Sebocyte culture medium