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axoCells™ human iPSC-derived sebocytes (unaffected), male donor, aged 30, ≥2 million cells, PCi-SEB_CAU
axoCells™ human iPSC-derived sebocytes (unaffected), male donor, aged 30, ≥2 million cells, PCi-SEB_CAU for dermatology research, pharmacology, toxicology and drug discovery, including 3D skin reconstruction.
Sebocytes made from iPSCs which were generated from a 30 year old male donor’s PBMCs.
- Derived from human iPSCs
- Express the typical markers of sebocytes, MUC1 and KRT7 and other functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R)
- Exhibit functional responses to testosterone and linoleic acid
Specifications
Number of cells ≥2 million
Donor Male, 30 years old
Genetics Healthy control
Description
axoCells™ human iPSC-derived sebocytes (unaffected), male donor, aged 30, ≥2 million cells, PCi-SEB_CAU for cosmetics research. Sebocytes made from iPSCs generated from a 30 year old Caucasian male donor’s PBMCs.
Key highlights include:
• Derived from human iPSCs
• Assay ready in 4 days
• Express the typical markers MUC1 and KRT7
• Exhibit functional response to testosterone and linoleic acid
• Can be used to fuel advanced in vitro models including 3D cell resconstruction, for dermatology research, toxicology and drug discovery
These cells are frequently used in in vitro cosmetics models, including acne models, for research, drug discovery and toxicology testing.
Top Resources
Additional Information
Phenotypic relevance: Morphology
PCI-SEB human iPSC-derived sebocytes exhibit typical epithelial morphology of primary sebocytes with heterogeneity in cell size due to lipid accumulation. Magnification
Phenotypic relevance: ICC
PCI-SEB sebocytes express the key markers MUC1 (MUCIN 1) and KRT7 (KERATIN 7).
Phenotypic relevance: flow cytometry
PCI-SEB sebocytes exhibit >90% purity as measured by KRT7+ expression. Black: isotype control ; Red: anti-KRT7 antibody.
Phenotypic relevance: RT-qPCR
Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 day.
Phenotypic relevance: RT-qPCR
Evolution of specific markers after 3 (d3) and 5 (d5) days in culture compared to primary keratinocytes (Ker). Functional markers such as lipid activated transcription factor PPARG-γ, FADS2 (fatty acid biosynthesis pathway), androgen receptors (AR) and melanocortin 5 receptor (MC5R) are strongly expressed after 5 day.
Functional relevance: response to testosterone
PCI-SEB sebocytes respond to a 96h treatment with testosterone (10 µM) by a 2-fold increase in lipid content. This response is significantly inhibited by the 5-α-reductase inhibitor finasteride (10 µM).
Applications
PCi-SEB Sebocytes have been specifically developed for use in advanced in vitro models of skin for cosmetic toxicology testing, research and drug discovery.
We have a community of pharma and cosmetics companies that use our sebocytes to build powerful in vitro models to investigate cosmetic toxicology and to model skin disorders (including acne) for drug discovery.
Quality
Guided by over a decade of expertise, we ensure confidence in the quality of our iPSC-derived cells with rigorous QC. All cells come with a full Certificate of Analysis to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into sebocytes for commercial use.
Patient samples used to create these sebocytes have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
Makrantonaki, E., Ganceviciene, R., & Zouboulis, C. (2011). An update on the role of the sebaceous gland in the pathogenesis of acne. Dermatoendocrinol, 3(1), 41-49. doi:10.4161/derm.3.1.13900
Pappas, A., Fantasia, J., & Chen, T. (2013). Age and ethnic variations in sebaceous lipids. Dermatoendocrinol, 5(2), 319-324. doi:10.4161/derm.2536
Xia, L., Zouboulis, C. C., & Ju, Q. (2009). Culture of human sebocytes in vitro. Dermatoendocrinol, 1(2), 92-95.
Media and Reagents
- Sebocyte culture medium