How to Videos and Guides

Easy step-by-step videos and guides to learn how to get the best out of our iPSC-derived cells

Neuroscience

Generation and Functional Characterization of hiPSC-Derived Cortical and Sensory Neurons

Generation and Functional Characterization of hiPSC-Derived Cortical and Sensory Neurons

How to prepare your culture vessels with SureBond

In this video, you will learn how to coat your culture vessels with Axol’s SureBond, this coating reagent is optimized for all of our Neural Culture Systems and is used during the expansion of early cultures, as it aids cell signaling and cell health during expansion.

How to precoat your culture vessels with ReadySet

In this video, you will learn how to coat your culture vessels with Axol’s ReadySet, this pre-coating reagent is optimized for all of our Neural Culture Systems and is used for final assay formats, as it is a strong cell adhesive and aids cell survival during multiple medium changes and long culture periods.

Cardiovascular

Generation and Functional Characterization of hiPSC-Derived Cardiomyocytes

Generation and Functional Characterization of hiPSC-Derived Cardiomyocytes

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How to use Microelectrode Array (MEA) to assess the electrophysiological properties of iPSC-derived Atrial and Ventricular Cardiomyocytes

Microelectrode array (MEA) is an easy-to-use, label-free and high-throughput method for identifying the electrical output from cells. MEA has many applications such as modelling network connectivity between neurons and arrhythmia in cardiomyocytes additionally, MEA is an ideal platform to investigate the effect of toxic compounds on the electrophysiology of cells.

How to seed atrial cardiomyocytes on Multi-Electrode Array (MEA)

In this video, you will learn how to prepare the MEA plate before seeding iPSC-derived Cardiomyocytes. We will walk you through setting up the cloning rings, coating with fibronectin and finally seeding cardiomyocytes on to the MEA plate. Before beginning download our handy guide on how to prepare Axol’s Cardiomyocyte Maintenance and Plating Medium, along with step by step details on how to thaw and culture the iPSC-derived Cardiomyocytes.

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