In this video, you will learn how to coat your culture vessels with Axol’s ReadySet, this pre-coating reagent is optimized for all of our Neural Culture Systems and is used for final assay formats.
How to precoat your culture vessels with ReadySet
How to prepare your culture vessels with ReadySet and SureBond
How to prepare your culture vessels with ReadySet and SureBond
In this video, you will learn how to coat your culture vessels with Axol’s ReadySet, this pre-coating reagent is optimized for all of our Neural Culture Systems and is used for final assay formats, as it is a strong cell adhesive and aids cell survival during multiple medium changes and long culture periods. Cells cannot be dissociated from culture vessels coated with ReadySet. SureBond must be used on top of ReadySet.
Step 1: Materials you will need
The day before thawing your cells thaw SureBond overnight at 4 degrees.
Step 2: Preparing the SureBond
Once thawed, dilute the SureBond in Dulbecco’s-PBS to a final concentration of 20ug/ml.
Step 3: Add SureBond
Add the SureBond to your culture vessel, we recommend a volume of 200 ul per cm^2.
Step 4: Rock the dish to distribute the SureBond
Rock the culture dish back and forth to evenly distribute the matrix.
Step 5: Incubate overnight
Incubate the culture vessel overnight at 37oC.
Step 6: Remove the SureBond
When you are ready to plate your cells remove the coating reagent. Do not let the culture vessel dry out and do not wash the dish.
