>>> READY TO SHIP PRODUCT
axoCells™ sensory neuron maximizer supplement, 1 ml
axoCells™ sensory neuron maximizer supplement, 1 ml is a fully defined media supplement that accelerates the maturation of iPSC-derived sensory neurons. Suitable for use with axoCells sensory neuron maintenance media.
• Fully-defined media supplement
• Accelerates the maturation of iPSC-derived sensory neurons
• Contains signaling factors found in the in vivo environment of sensory neurons
Specifications
Volume 1 ml
Shipping conditions Dry ice
Storage conditions -80°C
Description
axoCells Sensory Neuron Maximizer Supplement (1 ml) is a fully-defined media supplement that accelerates the maturation of iPSC-derived sensory neurons (ax0055, ax0555) for pain and sensation research. Suitable for use with axoCells Sensory Neuron Maintenance Media.
It works by mimicking in vivo signals between sensory neurons and their supporting cells. The supplement contains signaling factors present in the peripheral nervous system and in particular the native environment of sensory neurons. Utilizing this supplement accelerates the maturation of iPSC-derived sensory neurons (ax0055, ax0555) in vitro.
Top Resources
Additional Information
Phase contrast
Phase contrast images show the differentiation and maturation of axoCells Sensory Neuron Progenitors over two weeks after thawing and treatment with mitomycin C.
Phase contrast images of axoCells Sensory Neuron Progenitors The cells were plated on SureBond-XF in Neural Plating Medium. The cells were then treated with mitomycin C two days after thawing and cultured in the supplemented Sensory Neuron Maintenance Medium. (axoCells sensory neurons should be cultured for a minimum of 3 weeks prior to performing endpoint assays.)
Immunocytochemistry
Immunocytochemistry (ICC) image of axoCells sensory neurons shows expression of the key marker TUJ-1.
The cells were plated on SureBond-XF in Neural Plating Medium. The cells were then treated with mitomycin C two days after thawing and cultured in the supplemented Sensory Neuron Maintenance Medium. Sensory neurons should be cultured for a minimum of 3 weeks before performing endpoint assays.
Culturing axoCells sensory neurons with Maximizer supplement speeds up maturation.
Capsaicin response
axoCells sensory neurons were challenged with capsaicin at day 22. Recordings were carried out on a multi-electrode array (MEA) platform, with 90% of the neurons responding to capsaicin challenge with increased spike measurements.
Dose response for capsaicin was observed, demonstrating how the axoCells sensory neurons could be used as advanced in vitro models for drug discovery and cosmetic toxicity testing.
Applications
Our axoCellsTM sensory neurons have been specifically developed for use in microfluidics systems, organ-on-chip devices and for in vitro disease modeling.
We have a community of top ten biopharma and research institutes that use our sensory neurons to build powerful in vitro models for drug discovery and research.
Protocols
We offer a fully optimized cell culture system including tailored Sensory Maintenance Medium to promote the viability and maturation of sensory neurons for endpoint assays on glass or plastic.
Quality
We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.
All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into sensory neurons for commercial use.
Patient samples used to create these neurons have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Publications
The RNA-Binding Protein HuR Is Integral to the Function of Nociceptors in Mice and Humans- Kunder N,et al. 2022. J Neurosci. doi: 10.1523/JNEUROSCI.1630-22.2022. Epub 2022 Oct 21. PMID: 36270801; PMCID: PMC9761683.
Macrophage epigenetic memories of early life injury drive neonatal nociceptive priming- Dourson AJ et al. bioRxiv [Preprint]. doi: 10.1101/2023.02.13.528015.
SARS-CoV-2 papain-like protease activates nociceptors to drive sneeze and pain.- Mali et al 2024. bioRxiv [Preprint]. 2024 doi: 10.1101/2024.01.10.575114.
Emerging Neurotechnology for Antinoceptive Mechanisms and Therapeutics Discovery – Black et al 2018 Biosensors and Bioelectronic https://doi.org/10.1016/j.bios.2018.11.015
A Microfluidic Approach to Investigate Changes in Functional Properties of Nociceptive Axons Underlying Inflammatory Pain States – Natasha Rangwani 2018. PhD Thesis
Human stem cell derived sensory neurons are positioned to support varicella zoster virus latency – Sadaoka et al. 2020 BioRxiv https://doi.org/10.1101/2020.01.24.919290
Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency – Ouwendijk et al. 2020 Nature Communications https://doi.org/10.1038/s41467-020-20031-4
Mechanistic insights into the pathogenesis of microtubule-targeting agent-induced peripheral neuropathy from pharmacogenetic and functional studies – Chua et al. 2021 BCPT https://doi.org/10.1111/bcpt.13654
Considerations for a Reliable In Vitro Model of Chemotherapy-Induced Peripheral Neuropathy – Eldridge et al. 2021 Toxics https://doi.org/10.3390/toxics9110300
In Vitro Pain Assay Using Human iPSC-Derived Sensory Neurons and Microelectrode Array – Odawara et al. 2022 Toxicological Sciences https://doi.org/10.1093/toxsci/kfac045
Global analyses of mRNA expression in human sensory neurons reveals eIF5A as a conserved target for inflammatory pain – Chase et al. 2022 FASEB J. https://doi.org/10.1096%2Ffj.202101933RR
Use of a human induced pluripotent stem cell-derived dorsal root ganglion neurone model to study analgesics in vitro: proof of principle using lidocaine – Smulders et al. 2022 British Journal of Anaesthesia https://doi.org/10.1016/j.bja.2022.09.009
Chow SYA, et al. 2022. Human sensory neurons modulate melanocytes through secretion of RGMB. Cell Rep. 2022 Sep 20;40(12):111366. doi: 10.1016/j.celrep.2022.111366. PMID: 36130522.
Novel artificial nerve transplantation of human iPSC-derived neurite bundles enhanced nerve regeneration after peripheral nerve injury – Nishijima et al. 2023 Research Square https://doi.org/10.21203/rs.3.rs-3503996/v1
Scalable generation of sensory neurons from human pluripotent stem cells – Deng et al. 2023 Stem Cell Reports https://doi.org/10.1016/j.stemcr.2023.03.006
New human in vitro co-culture model of keratinocytes and sensory neurons like cells releasing substance P with an evaluation of the expression of ZIKV entry receptors: A potent opportunity to test Zika virus entry and to study Zika virus’ infection in neurons? – Bocciarelli et al. 2023 Experimental Dermatology https://doi.org/10.1111/exd.14870
Deng T, et al. 2023. Scalable generation of sensory neurons from human pluripotent stem cells. Stem Cell Reports. 2023 Apr 11;18(4):1030-1047. doi: 10.1016/j.stemcr.2023.03.006. PMCID: PMC10147831.
Media & Reagents
Ensure high-quality, mature sensory neurons in just 21 days with our fully optimized media Sensory Neuron Maximizer kit (ax0158) and Maturation Maximizer media supplement (ax0058). We also supply cells, all growth factors and coating materials as a kit Human iPSC-Derived Sensory Neuron Maximizer Kit (ax0157) with cells).
