Human iPSC-Derived Sensory Neuron Maximizer Kit
We have put together our hiPSC-Sensory Neuron Progenitors with our new optimized media including the MATURATION MAXIMIZER supplement with the required growth factors in one kit with an easy-to-follow protocol to help you generate the best sensory neuron culture for your research.
MATURATION MAXIMIZER is a fully defined medium supplement for enhancing the existing Sensory Neuron Maintenance Medium (ax0060). It works by mimicking in vivo signals between sensory neurons and their supporting cells. The supplement contains signaling factors present in the peripheral nervous system and in particular the native environment of sensory neurons. Utilizing this supplement accelerates the maturation of iPSC-derived sensory neurons in vitro.
iPSC-derived sensory neurons treated with the Maximizer express key markers, such as TRPv1, TRPa1, TRPv2, TRPv3 and TRPm8. Kinetic states of e.g. TRPv1 are in a fast response inward rectifying state, to agonists such as Capsaicin. Neuronal responses to capsaicin occur at 1μM for 90% of the sensory neuron population.
The MATURATION MAXIMIZER supplement makes the Human iPSC-derived sensory neuronal culture a more physiological relevant model.
Functional Characterization using MEA analysis
The MATURATION MAXIMIZER supplement significantly reduces the time to maturation and gives a more physiological relevant model with neuronal response to capsaicin and menthol after only 22 and 27 days, respectively.
Calcium Imaging of the iPSC-sensory neurons’ responses to 1 μM Capsaicin when cultured with and without Maximizer. When cultured with Sensory Neuron Maturation Maximizer Supplement, sensory neurons respond to low concentrations of Capsaicin indicating the presence of functional TRPV1 channels.
A. Average deltaF/Fo values for the unsupplemented and Maximizer-supplemented sensory neurons treated with vehicle control and 1 μM capsaicin on Day 20 of culture.
B & C. Pie Chart and Table showing capsaicin threshold concentrations for sensory neurons treated with and without Maximizer. 91% of Regions-Of-Interest (ROIs) show a response to the lowest concentration of capsaicin tested (1 μM) compared to only 19% of control cells and while 99% of the ROIs of treated sensory neurons respond to the highest concentration of capsaicin (100 μM), 57% of non-treated sensory neurons remain non-responsive.
This shows that even by Day 20, the Maximizer-treated sensory neurons show enhanced capsaicin responses compared to non-treated.