axoCellsTM Human iPSC-derived Microglia

Build high-quality neurodegenerative disease models in 7 days. 

Ensure confidence in your neural workflow with high-quality axoCellsTM microglia differentiated from human induced pluripotent stem cells (iPSCs). Derived from consenting donor fibroblasts, we’ve specifically designed our production method to equip you with physiologically relevant cells, by mimicking the in vivo pathway of development for brain-resident macrophages. 

With Axol, you can rely on physiologically relevant microglia that express the three key markers (IBA1, TMEM119, and P2RY12) and demonstrate robust functional activity, measured by cytokine release, phagocytosis, and chemotaxis. With our microglia, you can build robust mono- and co-culture models to investigate neurological diseases and drug discovery.  

Updates to protocol

Part Change Rationale Previous version New version
Coating Cell Culture Ware

 

Use PBS to wash coating off plates instead of sterile water Better adherence of cells Use water to wash Use PBS (-/-) to wash
Thawing axoCells Human iPSC-Derived Microglia

 

ROCKi (Y-27632) used at thaw instead of Thiazovivin Better thaw recovery Thiazovivin used at 10 mM  y-27632 used at 10 mM
Changed the wording around thaw process Emphasize process is done gently and quickly
Plating axoCells Human iPSC-Derived Microglia

 

Removed 20 min wait between plating cells and putting in incubator No need for this step. Does not add value Leave plates in the hood after seeding cells for 20min Place plates with cells in the incubator directly after thaw
Preparation of Reagents Addition of 2-mercaptoethanol to Complete Microglia Media 2-mercaptoethanol is unstable in solution so should be added immediately before use 2-mercaptoethanol was previously included in ax0660d but removed due to unstable nature Add 5mM 2-mercaptoethanol to Complete Microglia Media

**NOTE: only applies to media received Aug2025 or after**