Dermatology user protocols

View and download Axol’s validated user protocols for skin models

axoCells™ human iPSC derived sebocytes

Sensory Neuron Progenitors 2.0 (Xeno-Free)

Sensory Neuron Progenitor User Guide

Change Log

Protocol	Version / No.	Section	Change log	Rationale	Previous version	New version
Sensory Neuron Progenitors	COM/PRT/31 Version 1	Preparation of Media and Coating Reagents	SureBond-XF addition to Neural Plating Media	To aid cell adherance	No SureBond-XF addition	SureBond-XF added at a 1:400 dilution to Neural Plating Media. Once made the Neural Plating Media must be used immediately and cannot be refrozen.
		Preparation of Media and Coating Reagents	Mitomycin C concentration and reconsitution	Optimised dosing to prevent neuronal cell death	2.5µg/ml	2.0µg/ml
		Preparation of Media and Coating Reagents	Mitomycin C concentration and reconsitution	Optimised dosing to prevent neuronal cell death	Made up in DMSO	Made up in water (so needs to be made fresh each time)
		Preparation of Media and Coating Reagents	Lyophilized PDL changed to liquid PDL and change in concentration used	Enables improved cell adherence and plating consistency	Powdered PDL made up in water at and used at 0.5mg/ml	Liquid PDL diluted to 0.05mg/ml
		Growth Arrest and Purification	Mitomycin C removal – Change of wash solution	To aid removal of Mitomycin C	D-PBD (without calcium or magnesium)	Two washes in Sensory Neuron Maintenance Media
		Coating Cell Culture Ware	Coating incubation time and temperature	Enables improved cell adherence and plating consistency	Incubate plate coated with PDL for 5 mins at room temperature	Incubate plate coated with PDL for 60 mins at 37°C
		Differentiation of axoCells Sensory Neuron Progenitors	Seeding density	Optimised cell density based on new coating reagents	100,000/cm²	100,000 – 150,000/cm²

* NB. You can revert to the previous method and make up mitomycin C in DMSO (which can be stored for up to 4 weeks at -80°C) but performance in Axol’s hands is better when made up in water.