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Human iPSC-Derived Ventricular Cardiomyocyte Kit (Male)

$1,235.40
Product code:
SKU: ax2500

All Axol’s cardiomyocytes are derived from human iPSCs and ONLY human iPSCs. We do NOT use animal cells or embryonic stem cells (ESCs).

Axol Bioscience; Quality Cells, Consistent Results, a brand you can trust.

Description

Human iPSC-Derived Ventricular Cardiomyocyte Kit (Male)

Human iPSC-Derived Ventricular Cardiomyocyte Kit contains the optimal system of cells, tailored culture medium and optimized coating solution for maximal cell viability and growth.

Product Specification

Starting material Fibroblasts
Donor gender Male
Donor age at sampling 74 yrs
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & fully defined medium
Genetic modification None
Kit components 1 vial of Ventricular Cardiomyocytes (1 million cells), 1 bottle of Cardiomyocyte Maintenance Medium (500 mL) and 1 vial of Fibronectin Coating Solution (1 mL)
Growth properties Adherent
Disease information Healthy
Shipping conditions Dry ice
Storage conditions vapour phase nitrogen

Resources

Technical Resources

No results found.

References

Sutton K, Ridly J, El Haou S et al.

Human Stem Cell-Derived Cardiomyocytes: In Vitro Assays and Screening Platforms for Exploring Ventricular and Atrial Phenotypes Journal of Pharmacological and Toxicological Methods (2017)

Martella D, Paoli P, Pioner J.M et al.

Liquid Crystalline Networks toward Regenerative Medicine and Tissue Repair Small Wiley Online Library (2017)

Papp R, Bett GCL, Lis A et al.

Genomic upregulation of cardiac Cav1.2α and NCX1 by estrogen in women. BioMed Central (2017)

Zuppinger C, Gibbons G, Dutta-Passecker P et al.

Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes. European Journal of Histochemistry (2017)

Chang Y, Broyles CN, Brook FA et al.

Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics. PLoS One (2017)

Shen Y, Dana H, Abdelfattah A.S et al.

A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein Biorxiv (2018)

FAQS

Do you have any questions?

1. At the resuspension step, using P1000 and 1ml of plating medium to pipette up and down at least 5 times gently against the falcon tube which would partially disintegrate the aggregates; 2. In the counting step do not count the large aggregates and seed the culture vessel at a density of 50,000 cells/cm2 as instructed by the protocol; 3. The day after plating (Day 1), change Plating Medium to Cardiomocyte Maintenance Medium which would remove debris and cells that didn’t attach (including large aggregates). 4. Using the recommended seeding density we have achieved over 70% confluence culture after plating.

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