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axoCells™ human iPSC-derived motor neurons + microglia co-culture (unaffected) cells, media and supplement kit
The axoCells™ human iPSC-derived motor neuron and microglia media and supplement co-culture kit is an optimized culture system for co-culture of axoCells™ motor neurons and microglia, supporting ALS research, neuroinflammation studies, and compound screening.
- Complete co-culture kit containing human iPSC-derived motor neurons, microglia, media and supplements
- Supports ALS and MND research and enables investigation of neuron-microglia interactions
- Motor neurons express key markers including SMI32, Islet 1, ChAT, HB9 and βIII-tubulin (TUJ1)
- Microglia express key markers including IBA-1, TMEM119 and P2RY12 and demonstrate robust functional activity
Specifications
Number of cells ≥2 million cells, ≥1 million cells
Donor Male, 40-50-year-old
Genetics Healthy control
Description
The axoCells™ human iPSC-derived motor neuron and microglia media and supplement co-culture kit is a ready-to-use culture system optimized for co-culture of axoCells™ motor neurons and microglia. Supporting studies of ALS and other neurodegenerative diseases, the kit enables investigation of neuron-microglia interactions, neuroinflammation and the contribution of microglia to neurodegeneration, while providing a robust platform for disease modeling and compound screening. The kit contains axoCells™ motor neurons (ax0076), microglia (ax0664), media and supplements. Additional motor neurons and microglia can also be generated through our custom differentiation services.
Key features
- Complete co-culture kit containing human iPSC-derived motor neurons, microglia, media and supplements
- Supports ALS and MND research and enables investigation of neuron-microglia interactions
- Motor neurons express key markers including SMI32, Islet 1, ChAT, HB9 and βIII-tubulin (TUJ1)
- Microglia express key markers including IBA-1, TMEM119 and P2RY12 and demonstrate robust functional activity
Top Resources
Additional Information
Microglia and astrocytes are present in triculture at 21 days in culture
Confocal image of control motor neurons in monoculture (left) or in triculture with isogenic microglia and astrocytes at day 5 (right). Green = CD44, red = Iba1, Blue = Hoechst 33342, white = βII-tubulin
Timing of addition of microglia and astrocytes alters the burst activity of motor neurons in triculture
Co-culture of motor neurons from an unaffected donor with isogenic astrocytes and microglia increases burst strength of spontaneously firing motor neurons. Earlier addition induces earlier increases in burst amplitude.
A) Motor neuron monoculture, (B) motor neurons with astrocytes and microglia added late. N=3., (C) motor neurons with astrocytes and microglia added early. n=3 , (D) Glia added late, (E) Glia added early.
The axoCells™ human iPSC-derived motor neuron media and supplement co-culture kit is a ready-to-use culture system optimized for co-culture of axoCells™ motor neurons, astrocytes, and microglia. Supporting studies of ALS and other neurodegenerative diseases, the kit enables investigation of cell-cell interactions, neuroinflammation and the contribution of glial cells to neurodegeneration, while providing a robust platform for compound screening.
Protocols
Protocols for incorporating microglia into co- and tri-culture with neurons are available as well as for microglia in monoculture.
Quality
We maintain or exceed industry-level quality with our ISO:9001-accredited production facility, guided by our rigorous quality control procedures and decades of scientific experience.
All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into microglia for commercial use.
Patient samples used to create these cells have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.
Raniga K et al, 2024. Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes. doi:10.1016/j.stem.2024.01.007.
Suzanne Edavettal, et al 2022. Enhanced delivery of antibodies across the blood-brain barrier via TEMs with inherent receptor-mediated phagocytosis, Med, Volume 3, Issue 12, 2022, Pages 860-882.e15, ISSN 2666-6340, https://doi.org/10.1016/j.medj.2022.09.007.
Timothy J. Sargeant, Célia Fourrier 2023. Human monocyte-derived microglia-like cell models: A review of the benefits, limitations and recommendations, Brain, Behavior, and Immunity, Volume 107, 2023, Pages 98-109, ISSN 0889-1591, https://doi.org/10.1016/j.bbi.2022.09.015.
McKee Chloe G., Hoffos Madison, Vecchiarelli Haley A., Tremblay Marie-Ève 2023. Microglia: A pharmacological target for the treatment of age-related cognitive decline and Alzheimer’s disease. Frontiers in Pharmacology 14 2023. http://doi.org10.3389/fphar.2023.1125982
iPSC-derived cells & co-culture kits
The kit contains axoCells™ motor neurons (ax0076) and microglia (ax0664). More co-culture kits are available to purchase. ALS astrocytes and microglia can also be generated through our custom differentiation services.
