Our iPSC-derived astrocytes are cryopreserved for convenience, and they are assay-ready within 48 hrs. Once thawed, Axol’s astrocytes display all indicative astrocytic markers and will respond to glutamate stimulation, a key functional characteristic within 48-60 hrs. They also express markers showing that Axol’s Human iPSC-derived astrocytes are physiologically representative of human primary astrocytes.
Human iPSC-Derived Astrocytes
Astrocyte dysfunction has been implicated in a number of neurological conditions such as Alzheimer’s and Parkinson’s diseases, autism, amyotrophic lateral sclerosis (ALS), Rett syndrome and schizophrenia.
Axol’s Human iPSC-derived astrocytes offer a convenient and physiologically-relevant tool to study these cells as an isolated population or within co-cultures of other neural cells for analysis of complex central nervous system (CNS) circuitry.
Axol’s Human iPSC-derived astrocytes express glial markers such as glial fibrillary acidic protein (GFAP), S100b, AQP4, ALDHL1L and EAAT1.
They have also been further validated for expression of other relevant markers including Vimentin, Kir4.1 (potassium channel) and CD44.
These cells exhibit low expression of Nestin, a marker of neural stem cells, and are tested to have very low or negative expression for MAP2, a marker of neurons.