Human iPSC-Derived Motor Neuron Progenitors
Axol Human iPSC-Derived Motor Neuron Progenitors are derived from a healthy male donor.
Axol iPSCs were differentiated to motor neuron progenitors using a combination of small molecules to regulate multiple signalling pathways. Initial expansion of iPSC-Motor Neuron Progenitors is possible before the terminal differentiation to motor neurons.
Axol Human iPSC-Derived Motor Neurons have been characterised by ICC and the motor neurons after 14 days in culture express HB9, LIM3, OLIG2 and TUJ1. After a further 14 days they express choline acetyltransferase (ChAT) and islet-1 (ISL1).
|Donor age at sampling||74 yrs|
|Reprogramming method||Episomal vector|
|Induction method||Monolayer & chemically defined medium|
|Size||1 vial (≥2 million cells/ vial)|
|Kit components||1 vial of Motor Neuron Progenitors (1 vial ≥1.5 million cells/ vial)|
|Shipping conditions||Dry ice|
|Storage conditions||vapour phase nitrogen|
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Always filter the media after retinoic acid (RA) addition. Do not subject the RA aliquots to multiple freeze thaws RA is extremely light sensitive so protect the media with RA by wrapping the bottle/tube with foil.
For best results, use Surebond + Readyset for final plating.