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Melanocyte Culture Media, 100 ml, + Supplement (400 µL)
Melanocyte Culture Media (100 ml) optimized for the culture and maintenance of iPSC-derived Melanocytes for cosmetics research. Suitable for use with PCi-MEL_CAU_1M, PCi-MEL_AFR_1M, PCi-MEL_ASI_1M, PCi-MEL_ALB3_1M
- Optimized for the culture and maintenance of iPSC-derived melanocytes
Specifications
Volume 100ml
Shipping conditions – 20◦ C
Storage conditions – 20◦ C
Description
Melanocyte Culture Medium (100ml) used for culture and maintenance of iPSC-derived melanocytes. Suitable for use with PCi-MEL_CAU_1M, PCi-MEL_AFR_1M, PCi-MEL_ASI_1M, PCi-MEL_ALB3_1M
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Additional Information
Phenotypic relevance: Morphology
PCi-MEL melanocytes were cultured for 5 days. Representative brightfield images. X20 objective
Phenotypic relevance: ICC
Immunostaining of PAX3, MITF, PMEL17 and TYRP1 (green) in PCi-MEL cultured for 5 days. Nuclei were counterstained with Hoechst (blue). X20 objective
Phenotypic relevance: flow cytometry
PCi-MEL (red) and hiPSC (black) were cultured for 5 days, singled and stained with a PMEL17 antibody and analyzed by flow cytometry for PMEL17 protein expression. > 95% PMEL17+
PCi-MEL are a relevant model for tanning bioassays
Caucasian PCi-MEL were cultured for 5 days and treated for 4 days with a propigmenting reference or vehicle. Representative brightfield images. X20 objective (A). Melanin content of Asian and Caucasian PCi-MEL was extracted, dosed and normalized to cell count after treatment for 4 days with a propigmenting reference or vehicle (B). African PCi-MEL were cultured for 5 days and treated for 4 days with a depigmenting reference, a propigmenting reference or vehicle and melanin content was extracted, dosed and normalized to cell count (C). Error bars represent Standard Deviation. Unpaired t test compared to the basal control with *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001.
PCi-MEL are a relevant model for lightening bioassays
PCi-MEL with different phototypes were cultured for 5 days. Representative brightfield images. X20 objective .Scale bar 50 µm (A). Melanin content of Albino (ALB), Caucasian (CAU), Asian (ASI) and African (AFR) PCi-MEL was extracted, dosed and normalized to cell count (B). Albino (ALB), primary melanocytes (HEMn), Asian (ASI) and African (AFR) PCi-MEL were cultured for 5 days and treated for 4 days with a depigmenting reference or vehicle and melanin content was extracted, dosed and normalized to cell count (C). Error bars represent Standard Deviation. Unpaired t test compared to the vehicle control with *p<0.05 ****p<0.0001.
Applications
PCi-MEL melanocytes are tools for dermatology screening, research, pharmacology, and drug discovery.
We have a community of biopharma and cosmetics companies that use our melanocytes for cosmetics screening and research, using powerful in vitro models.
Contact support@axolbio.com for the Melanocytes Protocol.
Quality
Guided by over a decade of expertise, we ensure confidence in the quality of our iPSC-derived cells with rigorous QC. All cells come with a Cellular Analysis Report to ensure ethical and biological conformity for your peace of mind.
Licenses & Consent
Axol has obtained all relevant licenses for reprogramming donor samples into iPSCs and differentiating these into melanocytes for commercial use.
Patient samples used to create these sebocytes have been ethically sourced and consented for research and commercial use. All cells come with a full Certificate of Analysis and are officially certified by HPSCreg®, to ensure ethical and biological conformity for your peace of mind.