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Multielectrode array characterization of human iPSC-derived motor neuron disease model for ALS drug discovery

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Multielectrode array (MEA) characterization of human iPSC-derived motor neuron disease model for ALS drug discovery

A rapid 20-day MEA characterization protocol for hiPSC-derived motor neurons using Axol Bioscience’s axoCells motor neurons, motor neuron maturation accelerator supplement, and the Axion BioSystems Maestro Pro platform for ALS drug discovery and research.

Key findings:

  • Four human iPSC lines, 3 from donors with ALS and 1 unaffected donor, were successfully differentiated to functional motor neurons.
  • The use of the ‘Accelerator’ supplement to mimic in vivo like environment enabled assay ready, functional firing of motor neurons in ~10 days.
  • Used in conjunction with the Maestro Pro, the ”thaw-to-data” time was ~20 days.
  • Motor neurons derived from ALS donor iPSCs were morphologically different to those from unaffected donors.
  • Through MEA analysis, morphological differences corresponded to functional phenotype differences including the key attribute of hyperexcitability.