Poster - Generation and characterization of cryopreserved axoCells human iPSC-derived microglia
Abstract
Microglia are tissue resident macrophages, making up 10 15% of the cells in the brain. Their key role in homeostasis is to support neurons, through actions such as synaptic pruning, as well as responding to inflammatory signals. As microglia play an important role in neuroinflammation they are a key target for drug discovery.
Current models do not provide a physiologically relevant background or are limited by a finite amount of material, making it difficult to reliably produce a model that recapitulates the disease in a human background. Therefore, induced pluripotent stem cells (iPSCs), alongside robust differentiation methodologies can provide a human model with the large scale production required for drug discovery. Here, we show the initial characterization of cryopreserved axoCells
iPSC derived microglial cells and demonstrate their use for studying functional activity.
