Axol Bioscience has announced the Early Access release of two iPSC derived interneuron products for use in co-culture.
Interneurons are the ‘brakes’ of the central nervous system. Connections between neurons in the brain are finely tuned and any increased electrical activity is dampened down by these cells. In the neocortex, the location for functions including sensory perceptions, emotion and cognition, interneurons represent between 20 and 30% of neurons.
axoCellsTM cortical inhibitory interneuron progenitors are derived from human iPSCs. In their progenitor state, the cells express the important Nkx-2.1 protein, indicative of mature parvalbumin and somatostatin interneuron development. After 20 days of culture, the cells mature to become interneurons. They will display key markers including parvalbumin, somatostatin, tyrosine hydroxylase, PAX-6, MAP2, beta-tubulin and GABA receptor and GAD enzyme. At day 30, the mature interneurons display functional calcium imaging responses to glutamic acid and glycine
stimulation.
The interneuron products from two different donor lines can be found here:
ax0662 – male, 40-50 year old donor
About Early Access
Early Access describes products in late stage of development and we welcome feedback on these products from the scientific community. These products are available to purchase at a discounted package price and will be supplied with a feedback form. Note, in Early Access the products will not have been manufactured at our ISO accredited facility and final protocols, documentation and literature may still be development.
For more information or a quotation, email operations@axolbio.com
