Abstract
Photoreceptor outer segments (POS) play a crucial role in the process of vision, and their dysfunction is implicated in retinal degeneration and eventual vision
loss. Retinal organoids (ROs) have become a valuable tool in the study of retinal disease mechanisms and in drug discovery pertaining to their ability to recapitulate aspects of the human retina. Understanding the dynamics of POS development and consistency across multiple batches of ROs is crucial for their wider adoption and routine use. In this study we have assessed the dynamics, efficiency and batch-to-batch reproducibility of POS development across three batches of wildtype human iPSC-derived retinal organoids produced at large scale.
