Coating solution that provides an optimal surface when expanding Human iPSC-Derived Neural Stem Cells.
SureBond is a coating solution that provides an optimal surface when expanding Human iPSC-Derived Neural Stem Cells. It permits neural cells to be passaged with Unlock (ax0044).
Optimized Coating Solution for Neural Stem Cells and Neurons
SureBond coating solution provides an optimal surface for culturing iPSC-derived neural stem cells and neurons.
It should be used when expanding neural stem cells and when passaging of the cells with Unlock is required.
|Size||3 x 120 μL vials|
|Shipping conditions||Dry ice|
|Usage notes||Centrifuge the vial before opening.|
Do you have any questions?
We recommend SureBond-XF: Xeno-free coating reagent (Ax0053) in combination with Poly-D-Lysine (Sigma Aldrich, P7405)
Please make sure you change medium gently and avoid adding the medium from one side of the wells throughout the 5-6 weeks of culture. If the cells start to peel from the corners, it can be repaired by adding Surebond (ax0041) into your standard feeding media. Usually, we use 120 uL Surebond in 12 mL medium for a few days until the layer re-attach. This can be applied no matter what coating has been used.
For best results, use Surebond + Readyset for final plating.