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axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HET

From: $745.00
Product code:
SKU: ax0311-kit

Please be aware that Neural Plating Medium ax0033 (not Xeno-Free) may be needed for your specific order. Please be sure to include ax0033 if required.

Product Quantity

Neural Maintenance Media Kit

Neural Plating Medium

SureBond-XF

SureBond

Recombinant Human FGF2

Recombinant Human EGF

Description

axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HET

Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the G2019S mutation (GGC>AGC) in the LRRK2 gene. This line is homozygous for the G2019S mutation so both alleles contain the mutation. Click on the product images to see the data and further details.

The G2019S mutation in LRRK2 has been implicated in autosomal-dominant familial Parkinson’s disease with late onset (Fonzo et al., 2006, Thaler et al., 2009). The G2019S mutation increases the kinase activity of LRRK2 causing increased autophosphorylation and substrate phosphorylation that may affect neuronal cell health in Parkinson’s disease patients (West et al., 2005).

Product Specification

Starting material Dermal fibroblast
Donor gender Female
Donor age at sampling 64 yrs
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification Heterozygous for the LRRK2 G2019S mutation (GGC>AGC)
Genetic modification Contains a puromycin resistance cassette (intronic)
Size ≥1.5 million cells
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions Liquid nitrogen

References

Thaler A, Ash E, Gan-Or Z et al.

The LRRK2 G2019S mutation as the cause of Parkinson’s disease in Ashkenazi Jews. Journal of Neural Transmission (2009)

West AB, Moore DJ, Biskup S et al.

Parkinson’s disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity. Proc Natl Acad Sci USA (2005)

Di Fonzo A, Tassorelli C, De Mari M et al.

Comprehensive analysis of the LRRK2 gene in sixty families with Parkinson’s disease. Eur J Hum Genet (2006)

FAQS

Do you have any questions?

Plate the cells on Readyset + Surebond (ax0052)

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