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Human iPSC-Derived Neural Stem Cells (Male)

From: $745.00
Product code:
SKU: ax0011

Please be aware that the correct volume of Neural Plating Medium ax0033 (not Xeno-Free) is needed for your specific order volume of cells.

Product Quantity

Neural Maintenance Media Kit

Neural Plating Medium

SureBond-XF

SureBond

Recombinant Human FGF2

Recombinant Human EGF

498 in stock

Description

Axol Human iPSC-Derived Neural Stem Cells

Axol Human iPSC-Derived Neural Stem Cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions. The NSCs express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6, FOXG1 and nestin, and spontaneously form polarized neural tube-like rosette structures when plated as a monolayer in culture (see below). Additionally, Axol NSCs are capable of generating a spectrum of cerebral cortical excitatory and inhibitory neurons that are electrically active and have the ability to form functional synapses and circuits in vitro. After thawing and plating, the neural stem cells terminally differentiate to acquire mature electrophysiological properties, and form functional synaptic networks over a period of 40 ~ 50 days.
Axol NSCs are easy to differentiate to neurons or mixed neural cell types, following our protocols and using our tailored media and reagent bundles. A highly pure population of neurons can be generated from Axol NSCs following the synchronous differentiation protocol. Using our specialized coating reagents, neurons derived from Axol NSCs can be maintained in culture long-term (>1 year). NSCs are available from multiple donors to suit your research needs and have been characterized extensively.

Axol Human iPSC-Derived Neural Stem Cells

Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
HLA serotype A2 A3 B35(Bw6) B44(Bw4) Cw4 Cw5
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification None
Size ≥1.5 million cells
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions vapour phase nitrogen

References

A. Odawara, H. Katoh, N. Matsuda, I. Suzuki.

Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons, Biochemical and Biophysical Research Communications (2015).

FAQS

Do you have any questions?

Please switch to the Maintenance Medium-XF after the suggested period of Neural differentiation medium-XF treatment (see page 12 and 15 of the Human iPSC-Derived Neural Stem Cell Master Protocol below).

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