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axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM

axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM

From: $745.00

Product code:

SKU: ax0320

Please be aware that Neural Plating Medium ax0033 (not Xeno-Free) may be needed for your specific order. Please be sure to include ax0033 if required.

	Product	Quantity
	Neural Maintenance Media Kit Add for $446.25 each	Neural Maintenance Media Kit quantity
	Neural Plating Medium Add for $137.00 each	Neural Plating Medium quantity
	SureBond-XF Add for $552.00 each	SureBond-XF quantity
	SureBond Add for $279.00 each	SureBond quantity
	Recombinant Human FGF2 Add for $389.00 each	Recombinant Human FGF2 quantity
	Recombinant Human EGF Add for $205.00 each	Recombinant Human EGF quantity

Description

axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM

Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the R406W mutation (CGG>TGG) into the MAPT gene. This line is homozygous for the R406W mutation so both alleles contain the mutation. Click on the product images to see the data and further details.

The R406W mutation in MAPT has been implicated in familial frontotemporal dementia and parkinsonism (Hutton et al., 1998; Behnam et al., 2015) and has been seen in patients with clinical presentation resembling Alzheimer’s disease (Rademakers et al., 2003; Lindquist et al., 2008). The R406W mutation reduces the ability of MAPT/tau to bind to microtubules in vitro (Hong et al., 1998).

Product Specification

Starting material	Dermal fibroblast
Donor gender	Female
Donor age at sampling	64 yrs
Karyotype	Normal
Reprogramming method	Episomal vector
Induction method	Monolayer & chemically defined medium
Genetic modification	Homozygous for the MAPT R406W mutation (CGG>TGG)
Genetic modification	Contains a puromycin resistance cassette (intronic)
Size	≥1.5 million cells
Growth properties	Adherent
Shipping conditions	Dry ice
Storage conditions	Liquid nitrogen

References

Hutton M, Lendon CL, Rizzu P et al.

Association of missense and 5′-splice-site mutations in tau with the inherited dementia FTDP-17. Nature (1998)

Rademakers R, Dermaut B, Peeters K et al.

Tau (MAPT) mutation Arg406Trp presenting clinically with Alzheimer disease does not share a common founder in Western Europe. Human Mutation (2003)

Lindquist SG, Holm IE, Schwartz M et al.

Alzheimer disease-like clinical phenotype in a family with FTDP-17 caused by a MAPT R406W mutation. European Journal of Neurology (2008)

Hong M, Zhukareva V, Vogelsberg-Ragaglia V et al.

Mutation-specific functional impairments in distinct tau isoforms of hereditary FTDP-17. Science (1998)

Behnam M, Ghorbani F, Shin JH et al.

Homozygous MAPT R406W mutation causing FTDP phenotype: A unique instance of a unique mutation. Gene (2015)

FAQS

Do you have any questions?

Plate the cells on Readyset + Surebond (ax0052)

Please switch to the Maintenance Medium-XF after the suggested period of Neural differentiation medium-XF treatment (see page 12 and 15 of the Human iPSC-Derived Neural Stem Cell Master Protocol below).

We recommend SureBond-XF: Xeno-free coating reagent (Ax0053) in combination with Poly-D-Lysine (Sigma Aldrich, P7405)

Please make sure you change medium gently and avoid adding the medium from one side of the wells throughout the 5-6 weeks of culture. If the cells start to peel from the corners, it can be repaired by adding Surebond (ax0041) into your standard feeding media. Usually, we use 120 uL Surebond in 12 mL medium for a few days until the layer re-attach. This can be applied no matter what coating has been used.

We only recommend using SureBond-XF (Ax0053) in combination with Poly-D-Lysine (Sigma Aldrich, P7405).

We do not recommend expansion or re-freezing of the the NSCs. Axol cannot guarantee the viability of the iPSC-derived NSCs and functionality of the neurons derived after re-freezing or expansion.

At day 21, spontaneous synaptic activities should be detected, and day 35 synchronised burst firing should occur.

It is possible to achieve a 90% pure population of cerebral cortical neurons after terminal differentiation using Neural Differentiation-XF Medium (System B). Repeated expansion of the NSCs will increase the glial population and conversely decrease the neuronal population.

The ratio of deep to upper layer neurons will change with the number of days in culture. After 2 weeks in Neural Maintenance-XF Medium, approx. 60% of neurons express deep layer markers but this will decrease with length of time in culture. We would recommend spontaneous differentiation for over 40 days to see a large percentage of upper layer neurons.

Yes, Axol iPSC-Derived Cortical Neurons when co-cultured with astrocytes have been shown to respond to high frequency stimulation resulting in a change in spike frequency presenting as a depression of potentiation of network transmission.

We typically use PAX6, SOX2, Nestin, FOXG1, OTX, ASPM, N-cadherin and Ki67 to identify NSCs.

NeuN, TBR1, TUJ1, MAP2, GAD67, VGLUT1, Synaptophysin, CTIP2, CUX1 and BRN2 can be used to identify cerebral cortical neurons.

axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM

Neural Maintenance Media Kit

Neural Plating Medium

SureBond-XF

SureBond

Recombinant Human FGF2

Recombinant Human EGF

Description

axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM

Product Specification

References

Hutton M, Lendon CL, Rizzu P et al.

Rademakers R, Dermaut B, Peeters K et al.

Lindquist SG, Holm IE, Schwartz M et al.

Hong M, Zhukareva V, Vogelsberg-Ragaglia V et al.

Behnam M, Ghorbani F, Shin JH et al.

FAQS

Do you have any questions?

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