Neural stem cells – cerebral cortex

Axol’s cerebral cortical neural stem cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions.

Axol Human iPSC-Derived Neural Stem Cells express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6 and FOXG1. They spontaneously form polarized neural rosette structures when cultured as a monolayer. Additionally, Axol’s NSCs can generate cortical neurons that are electrically active and have the ability to form functional synapses and neural circuits in vitro.

Products

Human iPSC-derived neural stem cells from healthy donors

Human iPSC-derived neural stem cells from healthy donors

Human iPSC-derived neural stem cells from healthy donors

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Human iPSC-derived neural stem cells from healthy donors

Characterisation

Phase contrast

Axol iPSC-Derived Neural Stem Cells grown in Neural Expansion-XF Medium with the addition of EGF and FGF2.

Two hours after thawing.

Six hours after thawing.

Two days after thawing.

Immunofluorescence

Axol Human iPSC-Derived Neural Stem Cells

FOXG1/SOX2/nestin

PAX6/vimentin/DAPI

OTX/Ki67/DAPI

ASMP/Ki67/DAPI

ZO1/NCAD/DAPI

ZO1/NCAD/DAPI

Transcriptome

Gene expression profiling of neural stem cells by microarray

The full primary data sets generated for our transcriptome analysis are available from Gene Expression Omnibus.

Two days after thawing.

Two days after thawing.

Transfection

Transfection of cerebral cortical neural stem cells with ReadyFect

ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days post-transfection.

Neurite outgrowth

Live imaging of neurite outgrowth with IncuCyte and NeuroTrackTM by Essen Bioscience Ltd.

Competitor product comparison

Time-course comparison: N2a mouse neuroblastoma cell line, primary mouse cortical neurons (CTX), competitor’s iPSC-derived neurons & Axol’s cortical NSC-derived neurons

Live imaging data by Axol Bioscience

Patch clamp

Whole cell patch clamp recordings by Ole Paulsen’s Lab (University of Cambridge)

Whole cell patch clamp recordings for characterizing neuronal electrical properties by Dr Paul Charlesworth

Electrophysiological characterization of Axol’s iPSC-Derived Cerebral Cortical Neurons

Multi-electrode array (MEA)

Human iPSC-derived neurons may be effectively used for drug discovery. Our collaborators, Ikurou Suzuki and his team at Tohoku Institute of Technology in Japan used Alpha MED Scientific’s multi-electrod array (MEA) system to investigate the functional characteristics of human iPSC-derived neurons on their long-term spontaneous activity and drug responsiveness.

The results from this research, using Axol iPSC-Derived Cerebral Cortical Neurons and culture reagents, can be found in the Posters and Product Citations sections below.

Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).

Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).

Application of Axol iPSC-Derived Cerebral Cortical Neurons on multi-electrode arrays for studying network electrophysiology by Ole Paulsen’s Lab (University of Cambridge)

Complete MEA dataset by Dr Paul Charlesworth

Spontaneous spiking activity in Axol iPSC-derived neuronal cultures

Multi-electrode array studies of Axol iPSC-Derived Cerebral Cortical Neurons by Axion Biosystems

Dedicated protocols for using Axol’s neural stem cells on Axion MEAs and data generated with Axol’s neurons

An activity map from the AxIS Software suite showing positions of spike activity for each well of the MEA

iPSC-Derived Cerebral Cortical Neuron morphology on Axion MEAs

Transplantation

Transplantation of Axol iPSC-Derived Neural Stem Cells into neonatal rat cerebral cortex by Melissa Andrews’ lab (University of St Andrews)

Transplantation of Axol-iPSC Derived NSCs into neonatal rat cerebral cortex by Dr Melissa Andrews

Coronal sections from adult rat cortex six weeks after transplantation of Axol iPSC-Derived NSCs. Image shows human-specific NCAM (red).

Protocols & Application Notes

Posters

Product citations

Presentations

White Paper