Axol’s cerebral cortical neural stem cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions.
Axol Human iPSC-Derived Neural Stem Cells express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6 and FOXG1. They spontaneously form polarized neural rosette structures when cultured as a monolayer. Additionally, Axol’s NSCs can generate cortical neurons that are electrically active and have the ability to form functional synapses and neural circuits in vitro.
Products
Human iPSC-derived neural stem cells from healthy donors
Transfection of cerebral cortical neural stem cells with ReadyFect
ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days post-transfection.
Neurite outgrowth
Live imaging of neurite outgrowth with IncuCyte and NeuroTrackTM by Essen Bioscience Ltd.
Electrophysiological characterization of Axol’s iPSC-Derived Cerebral Cortical Neurons
Multi-electrode array (MEA)
Human iPSC-derived neurons may be effectively used for drug discovery. Our collaborators, Ikurou Suzuki and his team at Tohoku Institute of Technology in Japan used Alpha MED Scientific’s multi-electrod array (MEA) system to investigate the functional characteristics of human iPSC-derived neurons on their long-term spontaneous activity and drug responsiveness.
The results from this research, using Axol iPSC-Derived Cerebral Cortical Neurons and culture reagents, can be found in the Posters and Product Citations sections below.
Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).
Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).
Application of Axol iPSC-Derived Cerebral Cortical Neurons on multi-electrode arrays for studying network electrophysiology by Ole Paulsen’s Lab (University of Cambridge)
