Axol Biosciences Logo
Product has been added to your cart.
Contact
Products
Characterisation
Transcriptome
Transfection
Neurite outgrowth
Patch clamp
Multi-electrode array
Transplantation
Resources
  1. Home
  3. Shop
  5. Cardiomyocytes
  7. Neural stem cells – cerebral cortex

iPSC-Derived Neural Stem Cells

Neural stem cells – cerebral cortex

Axol’s cerebral cortical neural stem cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions.

Axol Human iPSC-Derived Neural Stem Cells express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6 and FOXG1. They spontaneously form polarized neural rosette structures when cultured as a monolayer. Additionally, Axol’s NSCs can generate cortical neurons that are electrically active and have the ability to form functional synapses and neural circuits in vitro.

Products

Human iPSC-derived neural stem cells from healthy donors

Characterisation

Phase contrast

Axol iPSC-Derived Neural Stem Cells grown in Neural Expansion-XF Medium with the addition of EGF and FGF2.

Two hours after thawing.

Six hours after thawing.

Two days after thawing.

Immunofluorescence

Axol Human iPSC-Derived Neural Stem Cells

FOXG1/SOX2/nestin

PAX6/vimentin/DAPI

OTX/Ki67/DAPI

ASMP/Ki67/DAPI

ZO1/NCAD/DAPI

ZO1/NCAD/DAPI

Transcriptome

Gene expression profiling of neural stem cells by microarray

The full primary data sets generated for our transcriptome analysis are available from Gene Expression Omnibus.

View full dataset

Two days after thawing.

Two days after thawing.

Transfection

Transfection of cerebral cortical neural stem cells with ReadyFect

ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days post-transfection.

Neurite outgrowth

Live imaging of neurite outgrowth with IncuCyte and NeuroTrackTM by Essen Bioscience Ltd.

Competitor product comparision

Time-course comparison: N2a mouse neuroblastoma cell line, primary mouse cortical neurons (CTX), competitor’s iPSC-derived neurons & Axol’s cortical NSC-derived neurons

Live imaging data by Axol Bioscience
Read Now

Patch clamp

Whole cell patch clamp recordings by Ole Paulsen’s Lab (University of Cambridge)

Whole cell patch clamp recordings for characterizing neuronal electrical properties by Dr Paul Charlesworth

Read Now

Electrophysiological characterization of Axol’s iPSC-Derived Cerebral Cortical Neurons

Multi-electrode array (MEA)

Human iPSC-derived neurons may be effectively used for drug discovery. Our collaborators, Ikurou Suzuki and his team at Tohoku Institute of Technology in Japan used Alpha MED Scientific’s multi-electrod array (MEA) system to investigate the functional characteristics of human iPSC-derived neurons on their long-term spontaneous activity and drug responsiveness.

The results from this research, using Axol iPSC-Derived Cerebral Cortical Neurons and culture reagents, can be found in the Posters and Product Citations sections below.

Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).

Read Now

Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).

Read Now

Application of Axol iPSC-Derived Cerebral Cortical Neurons on multi-electrode arrays for studying network electrophysiology by Ole Paulsen’s Lab (University of Cambridge)

Complete MEA dataset by Dr Paul Charlesworth

Read Now

Spontaneous spiking activity in Axol iPSC-derived neuronal cultures

Multi-electrode array studies of Axol iPSC-Derived Cerebral Cortical Neurons by Axion Biosystems

Dedicated protocols for using Axol’s neural stem cells on Axion MEAs and data generated with Axol’s neurons

Read Now

An activity map from the AxIS Software suite showing positions of spike activity for each well of the MEA

iPSC-Derived Cerebral Cortical Neuron morphology on Axion MEAs

Transplantation

Transplantation of Axol iPSC-Derived Neural Stem Cells into neonatal rat cerebral cortex by Melissa Andrews’ lab (University of St Andrews)

Transplantation of Axol-iPSC Derived NSCs into neonatal rat cerebral cortex by Dr Melissa Andrews

Read Now

Coronal sections from adult rat cortex six weeks after transplantation of Axol iPSC-Derived NSCs. Image shows human-specific NCAM (red).

Resources

Protocols & Application Notes
Posters
Product citations
Presentations
White Paper
Blogs

Protocols & Application Notes

Enriched Cerebral Cortical Neuron Protocol
Spontaneous Neuronal Differentiation Protocol
Data – APOE Genotyping of Axol NSCs and Astrocytes
In vitro Micro-electrode array assay for evaluating in vivo-like convulsive activity in hiPSC-derived neuron and astrocyte co-culture
Neurotoxicology screening using a high-sensitivity MEA platform

Posters

Report from multi-site pilot study of the HESI NeuTox Committee in collaboration with CSAHi and iNCENS: Detection of drug-induced seizure-like activities using MEA system in cultured human iPSC-derived neurons
Next-generation neurological disease models: isogenic tools for investigating frontotemporal dementia, Alzheimer’s and Parkinson’s diseases
Functional phenotypic characterization of iPSC-neurons from Alzheimer’s disease patients carrying PS-1 mutation in drug screening and disease modeling
Characterization and potential applications of human iPSC-derived neural stem cells
Modelling neurological disease: in vitro gene editing and iPSC differentiation combine to create powerful new tools
Functional maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture
Characterization and potential applications of human iPSC-derived neural stem cells
In vitro electrophysiological drug testing using human induced-pluripotent stem cells
Implementation of high throughput screening on iPS cells
Using iPSC-derived neural stem cells as a CNS model to study neuronal behaviour in development and neurodegernation
Modelling Alzheimer’s disease: Development of a scalable, high-throughput-compatible assay to detect tau aggregates using iPSC-derived cortical neurons maintained in a 3D-culture format

Product citations

Karikari T.K, Nagel D.A, Grainger A et al., Preparation of stable tau oligomers for cellular and biochemical studies Analytical Biochemistry (2018)
Kahn O.I, Schätzle P, van de Willige D et al., APC2 controls dendrite development by promoting microtubule dynamics Nature Communications (2018)
van Pel D.M, Harada K, Song D et al., Modelling glioma invasion using 3D bioprinting and scaffold-free 3D culture Journal of Cell Communication and Signaling (2018)
Nizou G, Wicks R.T, Wicks E.E et al., Human cortex spheroid with a functional blood brain barrier for high-throughput neurotoxicity screening and disease modeling Scientific Reports (2018)
Basuodan R, Basu A.P, Clowry G.J et al., Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo Journal of Anatomy – Wiley Online Library (2018)
Matsuda N, Odawara A, Katoh H et al., Detection of synchronized burst firing in cultured human induced pluripotent stem cell-derived neurons using a 4-step method Biochemical and Biophysical Research Communications (2018)
Rao A.N, Patil A, Brodnik Z.D et al., Pharmacologically increasing microtubule acetylation corrects stress-exacerbated effects of organophosphates on neurons. Traffic – Wiley Online Library (2017)
Zollo A, Allen Z, Rasmussen H et al., Sortilin-Related Receptor Expression in Human Neural Stem Cells Derived from Alzheimer’s Disease Patients Carrying the APOE Epsilon 4 Allele. Neural Plasticity (2017)
Gunhanlar N, Shpak G, van der Kroeg M et al., A simplified protocol for differentiation of electrophysiologically mature neuronal networks from human induced pluripotent stem cells. Molecular Psychiatry (2017)
Jones VC, Atkinson-Dell R, Verkhratsky A et al., Aberrant iPSC-derived human astrocytes in Alzheimer’s disease. Cell Death and Disease (2017)
Poulsen ET, Iannuzzi F, Rasmussen HF, et al., An aberrant phosphorylation of amyloid precursor protein tyrosine regulates its trafficking and the binding to the clathrin endocytic complex in neural stem cells of Alzheimer’s disease patients. Journal of Frontiers in Molecular Neuroscience (2017)
O’Rourke C, Lee-Reeves C, Drake RAL, et al., Adapting tissue-engineered in vitro CNS models for high-throughput study of neurodegeneration. Journal of Tissue Engineering (2017)
Wevers NR, van Vught R, Wilschut KJ, et al., High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform. Scientific Reports (2016)
Szabo A, Kovacs A, Riba J, et al., The endogenous hallucinogen and trace amine N,N-dimethyltryptamine (DMT) displays potent protective effects against hypoxia via sigma-1 receptor activation in human primary iPSC-derived cortical neurons and microglia-like immune cells. Frontiers in Neuroscience (2016)
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
Odawara A, Katoh H, Matsuda N, et al., Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. Scientific Reports (2016)
Medda X, Mertens L, Versweyveld S, et al., Development of a scalable, high-throughput-compatible assay to detect TAU aggregates using iPSC-derived cortical neurons maintained in a three-dimensional culture format. Journal of Biomolecular Screening (2016)
Cacabelos D, Ayala V, Granado-Serrano AB, et al., Interplay between TDP-43 and docosahexaenoic acid-related processes in amyotrophic lateral sclerosis. Neurobiology of Disease (2016)
Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015)
Verheyen A, Diels A, Dijkmans J, et al., Using human iPSC-derived neurons to model TAU aggregation. PLoS One (2015)
Lin L, Juan Y, Bjoern S, et al., In vitro differentiation of human neural progenitor cells into striatal GABAergic neurons. Stem Cells Translational Medicine (2015)

Presentations

Relevance of iPS cells in cell based screening and drug development
Frontotemporal dementia and Parkinson’s Disease cellular models: In vitro characterization of pathological phenotypes in gene-edited iPSC-derived neurons with MAPT and LRRK2 mutations
Abnormal phosphorylation of amyloid precursor protein tyrosine residues alters the APP trafficking in neurons from Alzheimer’s Disease affected patients
Nizou G, Wicks R.T, Wicks E.E et al., Human cortex spheroid with a functional blood brain barrier for high-throughput neurotoxicity screening and disease modeling Scientific Reports (2018)
Modeling FTDP-17 linked tauopathies and Alzheimer’s disease with human iPSC
Electrophysiological maturation and pharmacological responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture
The use of iPSC-derived cells and primary cells as in vitro models for toxicity screening
Modeling Alzheimer’s disease using stem cells
Rising to the challenges of human iPSC-derived cells for tox & drug screening

White Paper

Induced Pluripotent Stem Cells: recapitulating disease to facilitate drug discovery
Menu