Product Information
Human PBMCs from Patient Donors
| Catalog No. | Product Name | Product quantity | Short-term Storage | Long-term Storage | Thawing Instructions | ||||
| ax3417 | |||||||||
| ax3419 | |||||||||
| ax3420 | |||||||||
| ax3421 | PBMCs from Patient Donors | ≥10 million cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||||
| ax3422 | |||||||||
| ax3423 | |||||||||
| ax3424 | |||||||||
| Mononuclear Cell | Store at 4°C | Aliquot and | Thaw at 4°C or | ||||||
| ax3460 | Maintenance | 100 mL | for up to 1 | store at -20°C for up to 6 | at room | ||||
| Medium | month | months | temperature |
Lot-specific information such as donor details and passage number are stated in the Certificate of Analysis for each product.
Recommendations:
- Recommended culture vessel coating: Not required
- Recommended cell culture medium: Mononuclear Cell Maintenance Medium
- Recommended seeding density: As required (~1 million cells / mL)
- Recommended centrifugation speed: 400 x g for 10 minutes
Thawing & Plating:
- Transfer the vial of cells from liquid nitrogen storage with the vial buried in dry ice. Remove the vial from dry ice and transfer it immediately to a 37°C water bath.
- Thaw the cells quickly in a 37°C water bath until just prior to complete thawing.
- Wipe the outside of the vial with 70% ethanol.
- Gently resuspend the cells and transfer to a 15 mL sterile conical tube.
- Slowly add 10 mL of pre-warmed Mononuclear Cell Maintenance Medium.
- Rinse the cryovial twice with 1 mL of Mononuclear Cell Maintenance Medium to ensure all of the cells are transferred.
- Centrifuge the cells at 400 x g for 10 minutes.
- Carefully remove the supernatant and resuspend the cell pellet in 2 mL of pre-warmed Mononuclear Cell Maintenance Medium.
- Perform a cell count to determine the number of viable cells
- Dilute the cells into the required volume of pre-warmed Mononuclear Cell Maintenance Medium.
- Seed cells into the culture vessel at the required seeding density.
- Proceed with experiment assays.
- Incubate the cells at 37°C, 5% CO2 in a humidified incubator.
Passaging:
The PBMCs should be used directly for endpoint assays and should not be cultured long-term or passaged. The Mononuclear Cell Maintenance Medium is designed to maintain PBMCs after thawing and short-term culture. Expansion of PBMCs may lead to a loss of cell types and dominance of certain PBMC subtypes such as T cells. The expanded cells may also exhibit dependency on the particular cytokines and growth factors used.
Usage Statement
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or applications to humans.
