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Human Smooth Muscle Cell Protocol

Human Mesenchymal Stem Cells (Adipose Tissue Derived)

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Catalog. Product Name Format Short-term Long-term Thawing
No. Storage Storage Instructions
ax3070
Aortic Smooth
Muscle Cells
500,000 cells/vial Liquid Nitrogen Liquid Nitrogen See below
ax3071
Coronary Artery
Smooth Muscle Cells
500,000 cells/vial Liquid Nitrogen Liquid Nitrogen See below
ax3072
Pulmonary Artery
Smooth Muscle Cells
500,000 cells/vial Liquid Nitrogen Liquid Nitrogen See below
ax3073
Lung Smooth
Muscle Cells
500,000 cells/vial Liquid Nitrogen Liquid Nitrogen See below
ax3074
Bronchial/Tracheal
Smooth Muscle Cells
500,000 cells/vial Liquid Nitrogen Liquid Nitrogen See below
ax3075
Bladder Smooth
Muscle Cells
500,000 cells/vial Liquid Nitrogen Liquid Nitrogen See below
ax3076
Uterine Smooth
Muscle Cells
500,000 cells/vial Liquid Nitrogen Liquid Nitrogen See below
Smooth Muscle
ax3080 Cell Culture 500 mL
Store at 4°C for up to
Aliquot and store at
-20°C for up to
Thaw at 4°C or room
Medium
1 month
6 months
temperature

Lot-specific information such as donor details and passage number are stated in the Certificate of Analysis for each produc

Recommendations:

  • Recommended culture vessecoating: Not required
  • Recommended celculture medium: Smooth Muscle CelCulture Medium
  • Recommended seeding density: 2,500-5,000 viable cells/cm2
  • Recommended centrifugation speed: 200 x g for 5 minutes

Always count the number of viable cells after thawing

Thawing & Plating:

  • Transfer the viaof cells from liquid nitrogen storage with the viaburied in dry ice. Remove the viafrom dry ice and transfer it immediately to a 37°C water bath.
  • Thaw the cells quickly in a 37°C water bath untijust prior to complete thawing.
  • Wipe the outside of the viawith 70% ethanol.
  • Gently resuspend the cells and take an aliquot to perform a celcount.
  • Immediately after thawing, slowly dilute the cells into the required volume of pre-warmed Smooth Muscle Cell
  • Culture Medium (must be at least 10 mso that the concentration of DMSO is less than 1%).
  • Rinse the cryoviawith 1 mof Smooth Muscle CelCulture Medium to ensure alof the cells are transferred.
  • Seed cells into the culture vesseat the recommended seeding density of 2,500-5,000 viable cells/cm2.
  • Incubate the cells at 37°C, 5% CO2 in a humidified incubator.
  • Once the cells have attached (after 6-24 hours), replace the culture medium with fresh pre-warmed Smooth Muscle CelCulture Medium.
  • Frequency of media changes: Every 2 days

Passaging:

  • Passage when the culture reaches: 80-90% confluent
  • Recommended passaging reagent: Trypsin-EDTA
  • After adding passaging reagent, incubate the cells for 5 minutes at 37°C. Observe the cells at regular intervals for detachment from the culture vessel.
  • Once the cells have detached, dilute out the trypsin with pre-warmed Smooth Muscle CelCulture Medium.
  • Centrifuge the cells at 200 x g for 5 minutes.
  • Remove the supernatant and resuspend the celpellet in 1-2 mof pre-warmed Smooth Muscle CelCulture Medium.
  • Perform a celcount to determine the number of viable cells.
  • Dilute the cells into the required volume of pre-warmed Smooth Muscle CelCulture Medium.
  • Seed cells into the culture vesseat the recommended seeding density of 2,500-5,000 viable cells/cm2.
  • Incubate the cells at 37°C, 5% CO2 in a humidified incubator.

Usage Statement

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of
consumption or application to humans

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