Human Mesenchymal Stem Cells (Adipose Tissue Derived)
Catalog. | Product Name | Format | Short-term | Long-term | Thawing |
No. | Storage | Storage | Instructions | ||
ax3070 | |||||
Aortic Smooth | |||||
Muscle Cells | |||||
500,000 cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||
ax3071 | |||||
Coronary Artery | |||||
Smooth Muscle Cells | |||||
500,000 cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||
ax3072 | |||||
Pulmonary Artery | |||||
Smooth Muscle Cells | |||||
500,000 cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||
ax3073 | |||||
Lung Smooth | |||||
Muscle Cells | |||||
500,000 cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||
ax3074 | |||||
Bronchial/Tracheal | |||||
Smooth Muscle Cells | |||||
500,000 cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||
ax3075 | |||||
Bladder Smooth | |||||
Muscle Cells | |||||
500,000 cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||
ax3076 | |||||
Uterine Smooth | |||||
Muscle Cells | |||||
500,000 cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||
Smooth Muscle | |||||
ax3080 | Cell Culture | 500 mL | |||
Store at 4°C for up to | |||||
Aliquot and store at | |||||
-20°C for up to | |||||
Thaw at 4°C or room | |||||
Medium | |||||
1 month | |||||
6 months | |||||
temperature |
Lot-specific information such as donor details and passage number are stated in the Certificate of Analysis for each produc
Recommendations:
- Recommended culture vessecoating: Not required
- Recommended celculture medium: Smooth Muscle CelCulture Medium
- Recommended seeding density: 2,500-5,000 viable cells/cm2
- Recommended centrifugation speed: 200 x g for 5 minutes
Always count the number of viable cells after thawing
Thawing & Plating:
- Transfer the viaof cells from liquid nitrogen storage with the viaburied in dry ice. Remove the viafrom dry ice and transfer it immediately to a 37°C water bath.
- Thaw the cells quickly in a 37°C water bath untijust prior to complete thawing.
- Wipe the outside of the viawith 70% ethanol.
- Gently resuspend the cells and take an aliquot to perform a celcount.
- Immediately after thawing, slowly dilute the cells into the required volume of pre-warmed Smooth Muscle Cell
- Culture Medium (must be at least 10 mso that the concentration of DMSO is less than 1%).
- Rinse the cryoviawith 1 mof Smooth Muscle CelCulture Medium to ensure alof the cells are transferred.
- Seed cells into the culture vesseat the recommended seeding density of 2,500-5,000 viable cells/cm2.
- Incubate the cells at 37°C, 5% CO2 in a humidified incubator.
- Once the cells have attached (after 6-24 hours), replace the culture medium with fresh pre-warmed Smooth Muscle CelCulture Medium.
- Frequency of media changes: Every 2 days
Passaging:
- Passage when the culture reaches: 80-90% confluent
- Recommended passaging reagent: Trypsin-EDTA
- After adding passaging reagent, incubate the cells for 5 minutes at 37°C. Observe the cells at regular intervals for detachment from the culture vessel.
- Once the cells have detached, dilute out the trypsin with pre-warmed Smooth Muscle CelCulture Medium.
- Centrifuge the cells at 200 x g for 5 minutes.
- Remove the supernatant and resuspend the celpellet in 1-2 mof pre-warmed Smooth Muscle CelCulture Medium.
- Perform a celcount to determine the number of viable cells.
- Dilute the cells into the required volume of pre-warmed Smooth Muscle CelCulture Medium.
- Seed cells into the culture vesseat the recommended seeding density of 2,500-5,000 viable cells/cm2.
- Incubate the cells at 37°C, 5% CO2 in a humidified incubator.
Usage Statement
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of
consumption or application to humans