Product Information

Catalog. No. Product Name Format Stock Conc. Storage on Arrival Thawing Instructions Storage Once Thawed
ax3701 ARE Hepatocytes 35 million cells/vial N/A Dry Ice Follow protocol Liquid nitrogen
ARE Hepatocytes,
ax3702 CYP2D6 35 million cells/vial N/A Dry Ice Follow Liquid nitrogen
Overexpressing protocol
ARE Hepatocyte
ax3705 Thawing Medium 50 mL N/A Blue Ice N/A 4°C for 3 months
ARE Hepatocyte 4°C for 3
ax3710 Maintenance Medium + Supplement 500 mL N/A Blue Ice N/A months

Lotspecific information such as donor details and passage number are stated in the Certificate of Analysis for each product.

Recommendations:

  • Recommended culture vessel coating: Not required
  • Recommended cell culture medium: Axol Pericyte Growth Medium
  • Recommended seeding density: 4,000 viable cells/cm2
  • Recommended centrifugation speed: 220 x g for 5 min

Always count the number of viable cells after thawing

Thawing & Plating:

  • Thaw the cells quickly in a 37°C water bath until just prior to complete thawing
  • Wipe the outside of the vial with 70% ethanol
  • Gently resuspend the cells and transfer to a 15 mL sterile conical tube
  • Slowly add 10 mL of pre-warmed Pericyte Growth Medium
  • Rinse the cryovial with 1 mL of Pericyte Growth Medium to ensure all of the cells are transferred
  • Centrifuge the cells at 220 x g at room temperature for 5 min
  • Carefully remove the supernatant and resuspend in 1-2 mL of pre-warmed Pericyte
  • Growth Medium and perform a cell count
  • Dilute the cells into the required volume of pre-warmed Pericyte Growth Medium
  • Seed cells into the culture vessel at the recommended seeding density
  • After 24 h, replace the culture medium with fresh, pre-warmed Pericyte Growth Medium
  • Frequency of media changes: Every 2-3 days

Passaging:

  • Passage when the culture reaches: 80% confluent
  • Recommended passaging reagent: Trypsin-EDTA
  • Neutralize the trypsin with pre-warmed Pericyte Growth Medium and centrifuge the cells at 220 x g for 5 min
  • Remove the supernatant and resuspend in 1-2 mL of pre-warmed Pericyte Growth Medium
  • Perform a cell count to determine the number of viable cells
  • Dilute the cells into the required volume of pre-warmed Pericyte Growth Medium
  • Seed cells into the culture vessel at the recommended seeding density

We recommend using the human pericytes for endpoint assays prior to passage 4

Usage Statement

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans.

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