Human Corneal Epithelial Cells
| Catalog. | Product Name | Format | Short-term | Long-term Storage | Thawing |
| No. | Storage | Instructions | |||
| ax3502 | |||||
| Corneal Epithelial | |||||
| Cells | |||||
| 500,000 cells/vial | Liquid Nitrogen | Liquid Nitrogen | See below | ||
| ax3533 | |||||
| Corneal Epithelial | |||||
| Cell Culture Medium | |||||
| 500 mL | 4oC for 1 month | -20oC for 6 months | Thaw at 4oC or RT | ||
| ax0044 | Unlock | 25 mL | |||
| Aliquot and store at | |||||
| -80oC | |||||
| -80oC | Thaw at 4oC |
Lot-specific information such as donor details and passage number are stated in the Certificate of Analysis for each product.
Recommendations
- Recommended culture vessel coating: Not required
- Recommended cell culture medium: Corneal Epithelial Cell Culture Medium
- Recommended seeding density: 4,000 viable cells/cm2
- Recommended centrifugation speed: 200 x g for 5 minutes
Important! Always count the number of viable cells after thawing
Thawing and Plating
- Transfer the viaof cells from liquid nitrogen storage with the viaburied in dry ice. Remove the viafrom dry ice and transfer it immediately to a 37°C water bath.
- Thaw the cells quickly in a 37ºC water bath untijust prior to complete thawing.
- Wipe the outside of the viawith 70% ethanol.
- Gently resuspend the cells and take an aliquot to perform a celcount.
- Immediately after thawing, slowly dilute the cells into the required volume of pre-warmed CorneaEpitheliaCell
- Culture Medium (must be at least 10 mso that the concentration of DMSO is less than 1%).
- Rinse the cryoviawith 1 mof CorneaEpitheliaCelCulture Medium to ensure alof the cells are transferred.
- Seed cells into the culture vesseat the recommended seeding density of 4,000 viable cells/cm2.
- Incubate the cells at 37°C, 5% CO2 in a humidified incubator.
- Once the cells have attached (after 6-24 hours), replace the culture medium with fresh, pre-warmed Corneal EpitheliaCelCulture Medium.
- Frequency of media changes: Every 2 days
Passaging
- Passage when the culture reaches: 70-80% confluent
- Recommended passaging reagent: Unlock
- After adding passaging reagent, incubate the cells for 5 minutes at 37°C. Observe the cells at regular intervals for detachment from the culture vessel.
- Once the cells have detached from the culture vessel, dilute out the passaging reagent with CorneaEpitheliaCelCulture Medium.
- Centrifuge the cells at 200 x g for 5 minutes.
- Remove the supernatant and resuspend in 1-2 mof pre-warmed CorneaEpitheliaCelCulture Medium.
- Perform a celcount to determine the number of viable cells.
- Dilute the cells into the required volume of pre-warmed CorneaEpitheliaCelCulture Medium.
- Seed cells into the culture vesseat the recommended seeding density of 4,000 viable cells/cm2.
- Incubate the cells at 37°C, 5% CO2 in a humidified incubator.
Important! It is important that the cells are centrifuged in order to remove the passaging reagent before plating the Corneal Epithelial Cells
Usage Statement
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans.
