Cortical Neural Induction Kit – Product Information

Catalog. No. Product Name Stock Conc. Storage on Arrival Thawing Instructions Storage Once Thawed
Cortical Neural Induction Kit – Medium A 125 mL 1x Aliquot & store at -80°C for up to 6 months Overnight at 4°C Once thawed store aliquot at 4ºC for up to 1 week
Cortical Neural Induction Kit – Medium B 125 mL 1x Aliquot & store at -80°C for up to 6 months Overnight at 4oC Once thawed store aliquot at 4ºC for up to 1 week
Unlock (ax0044) 25 mL 1x Aliquot & store at -80°C for up to 6 months Overnight at 4°C Once thawed store aliquot at 4ºC for up to 1 week

Additional Reagents

Product Name Provider Catalog. No.
Essential 8™ Medium Thermo Fisher Scientific A1517001
Y-27632 2HCl (ROCK inhibitor) Selleck Chemicals S1049
Vitronectin (VTN-N) Recombinant Human Protein, Truncated Thermo Fisher Scientific A14700

The Cortical Neural Induction Kit volumes are sufficient for induction of two T25 flasks of pluripotent stem cells.

Important!
Axol Cortical Neural Induction Media DO NOT contain antibiotics or antifungal agents. Axol Bioscience does not recommend the use of antimicrobial agents such as penicillin, streptomycin and  amphotericin. Antimicrobial agents should not be necessary if proper aseptic technique is adopte

Preparation of Reagents

Cortical Neural Induction Kit – Medium A

  • Upon receipt, aliquot and store Medium A at -80°C protected from light.
  • When ready to use, thaw an aliquot of Medium A overnight at 4°C in the dark.
  • Avoid multiple freeze-thaw cycles.
  • Minimize the exposure to light.

Cortical Neural Induction Kit – Medium B

  • Upon receipt, aliquot and store Medium B at -80°C protected from light.
  • When ready to use, thaw an aliquot of Medium B overnight at 4°C in the dark.
  • Avoid multiple freeze-thaw cycles.
  • Minimize the exposure to light.

Cortical Neural Induction Kit – Coating Reagent

  • Upon receipt, aliquot and store Vitronectin (VTN-N) following the supplier’s instructions.
  • Axol recommend to dilute the Vitronectin (VTN-N) 1:50 in Dulbecco’s-PBS (1x) (D-PBS without calcium or magnesium) to make a 1x working solution (e.g. 120 μL Vitronectin (VTN-N) in 6 mL of D-PBS).
  • DO NOT VORTEX the Vitronectin (VTN-N).
  • Pre-coat culture vessels with the working solution of Vitronectin (VTN-N). We recommend coating a T25 flask with
    6 mL of 1x working solution.
  • Incubate at 37ºC, overnight.

Unlock

  • Upon receipt aliquot and store Unlock at -80°C protected from light. Stored at -80°C the reagent is stable for
    6 months from date of shipment