Human Immortalized Bladder Epithelial Progenitors
Catalog No.Product NameProduct quantity
Short-term Storage
Long-term Storage
Thawing Instructions
Short-term Storage
Long-term Storage
Thawing Instructions
| ax3008 | Immortalized Bladder | 500,000 | ||||||
| Epithelial Progenitors | cells/vial | |||||||
| Liquid nitrogen | Liquid nitrogen | See below | ||||||
| ax0039 | ||||||||
| Bladder Epithelial Cell | ||||||||
| Culture Medium | 500 mL | |||||||
| 4°C for 1 | -20°C for 6 | Thaw at 4°C or | ||||||
| month | months | RT | ||||||
| ax0044 | Unlock | 25 mL | Aliquot and | -80oC | Thaw at 4oC | |||
| store at -80oC |
Lot-specific information such as donor details and passage number are stated in the Certificate of Analysis for each product.
Recommendations:
- Recommended culture vessel coating: Not required
- Recommended cell culture medium: Bladder Epithelial Cell Culture Medium
- Recommended seeding density: 4,000 viable cells/cm2
- Recommended centrifugation speed: 200 x g for 5 minutes
Always count the number of viable cells after thawing
Thawing & Plating:
- Transfer the vial of cells from liquid nitrogen storage with the vial buried in dry ice. Remove the vial from dry ice and transfer it immediately to a 37°C water bath.
- Thaw the cells quickly in a 37°C water bath until just prior to complete thawing.
- Wipe the outside of the vial with 70% ethanol.
- Gently resuspend the cells and take an aliquot to perform a cell count.
- Immediately after thawing, slowly dilute the cells into the required volume of pre-warmed
- Bladder Epithelial Cell Culture Medium (must be at least 10 mL so that the concentration of DMSO is less than 1%).
- Rinse the cryovial with 1 mL of Bladder Epithelial Cell Culture Medium to ensure all of the cells are transferred.
- Seed cells into the culture vessel at the recommended seeding density of 4,000 viable cells/cm2 .
- Incubate the cells at 37°C, 5% CO2 in a humidified incubator.
- Once the cells have attached (after 6-24 h), replace the culture medium with fresh, pre-warmed Bladder Epithelial Cell Culture Medium.
- Frequency of media changes: Every 2 days
