Human Immortalized Bladder Epithelial Progenitors

Catalog No.Product NameProduct quantity
Short-term Storage
Long-term Storage
Thawing Instructions

ax3008 Immortalized Bladder 500,000
Epithelial Progenitors cells/vial
Liquid nitrogen Liquid nitrogen See below
Bladder Epithelial Cell
Culture Medium 500 mL
4°C for 1 -20°C for 6 Thaw at 4°C or
month months RT
ax0044 Unlock 25 mL Aliquot and -80oC Thaw at 4oC
store at -80oC

Lot-specific information such as donor details and passage number are stated in the Certificate of Analysis for each product.


  • Recommended culture vessel coating: Not required
  • Recommended cell culture medium: Bladder Epithelial Cell Culture Medium
  • Recommended seeding density: 4,000 viable cells/cm2
  • Recommended centrifugation speed: 200 x g for 5 minutes

Always count the number of viable cells after thawing

Thawing & Plating:

  • Transfer the vial of cells from liquid nitrogen storage with the vial buried in dry ice. Remove the vial from dry ice and transfer it immediately to a 37°C water bath.
  • Thaw the cells quickly in a 37°C water bath until just prior to complete thawing.
  • Wipe the outside of the vial with 70% ethanol.
  • Gently resuspend the cells and take an aliquot to perform a cell count.
  • Immediately after thawing, slowly dilute the cells into the required volume of pre-warmed
  • Bladder Epithelial Cell Culture Medium (must be at least 10 mL so that the concentration of DMSO is less than 1%).
  • Rinse the cryovial with 1 mL of Bladder Epithelial Cell Culture Medium to ensure all of the cells are transferred.
  • Seed cells into the culture vessel at the recommended seeding density of 4,000 viable cells/cm2 .
  • Incubate the cells at 37°C, 5% CO2 in a humidified incubator.
  • Once the cells have attached (after 6-24 h), replace the culture medium with fresh, pre-warmed Bladder Epithelial Cell Culture Medium.
  • Frequency of media changes: Every 2 days