Cord Blood CD34+ Cells
3.2 x 10e6
axoCells Human iPSC-Sensory Neuron Progenitors are derived from integration-free iPSCs and have been differentiated to neurons using small molecules. We also offer a fully optimized cell culture system including tailored Sensory Neuron Maintenance Medium and coating reagents to promote the viability and maturation of sensory neurons for endpoint assays on glass or plastic.
Our axoCells sensory neurons express several voltage-gated sodium ion channels and transient receptor potential (TRP) ion channels that play a key role in nociception. These include sodium ion channels Nav1.7 and the DRG-specific, TTX-resistant channels, Nav1.8 and Nav1.9 as well as the temperature-sensitive, TRPV1 and TRPM8, and TRPA1, a sensor of pungency, bitterness and cold.
axoCells Sensory Neuron Progenitors are available in large batch sizes for reliable and consistent results in high-throughput screening assays. The cells are also suitable for investigating disorders of the peripheral nervous system and chronic pain as well as drug targets for pain relief.
Please note: Axol recommends Poly-D-lysine, available from Sigma-Aldrich Cat. No. P7405, as an additional coating substrate for long term culture of the sensory neurons.
Phase contrast images show the differentiation and maturation of axoCells Sensory Neuron Progenitors over two weeks after thawing and treatment with mitomycin C.
Phase contrast images of axoCells Sensory Neuron Progenitors The cells were plated on SureBond-XF in Neural Plating Medium. The cells were then treated with mitomycin C two days after thawing and cultured in the supplemented Sensory Neuron Maintenance Medium. (axoCells sensory neurons should be cultured for a minimum of 3 weeks prior to performing endpoint assays.)
Sodium channel RNA expression analysis by cDNA PCR
axoCells Sensory Neuron Progenitors show RNA expression of all three voltage-gated sodium ion channels, Nav1.7, Nav1.8 and Nav1.9.
cDNA from axoCells Sensory Neurons was compared to cDNA from human tissue from the dorsal root ganglion (DRG). PCR analysis (40 cycles; 55oC ) confirmed the mRNA expression of SCN9A (82 bp, hNav1.7), SCN10A (149 bp, hNav1.8) and SCN11A (464 bp, hNav1.9) in axoCells sensory neurons. SCN5a (237 bp, hNav1.5) was included as a negative control. Data provided by Dr Edward Emery (University College London).
Nociceptive sensory neurons are unique in that they contain voltage-gated inward current sodium channels (Nav 1.8 and Nav 1.9) that are resistant to tetrodotoxin (TTX). The presence of these TTX-resistant ion channels was confirmed in axoCells sensory neurons.
Electrophysiological characterization of axoCells Sensory Neurons using patch clamp. A) Phase contrast image of axoCells sensory neurons; B) Example of a sodium-current elicited by a voltage step from -100 mV to -25 mV in the presence of tetrodotoxin (0.5 mM); C) Current-voltage plot of averaged Na-currents recorded from iPSC-derived sensory neurons in the presence of TTX (n=9). Data provided by Dr Edward Emery (University College London).