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iPSC-derived sensory neurons

We’ve used our expertise in neural differentiation to bring you human dorsal root ganglion (DRG) neurons derived from induced pluripotent stem cells (iPSCs).

axoCells Human iPSC-Sensory Neuron Progenitors are derived from integration-free iPSCs and have been differentiated to neurons using small molecules. We also offer a fully optimized cell culture system including tailored Sensory Neuron Maintenance Medium and coating reagents to promote the viability and maturation of sensory neurons for endpoint assays on glass or plastic.

Our axoCells sensory neurons express several voltage-gated sodium ion channels and transient receptor potential (TRP) ion channels that play a key role in nociception. These include sodium ion channels Nav1.7 and the DRG-specific, TTX-resistant channels, Nav1.8 and Nav1.9 as well as the temperature-sensitive, TRPV1 and TRPM8, and TRPA1, a sensor of pungency, bitterness and cold.

axoCells Sensory Neuron Progenitors are available in large batch sizes for reliable and consistent results in high-throughput screening assays. The cells are also suitable for investigating disorders of the peripheral nervous system and chronic pain as well as drug targets for pain relief.

Products

axoCells

Human NT-3

SKU: ax139811 (10 µg)

Recombinant Human Neurotrophin-3

10 μg / 100 μg / 500 μg / 1 mg

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Please note: Axol recommends Poly-D-lysine, available from Sigma-Aldrich Cat. No. P7405, as an additional coating substrate for long term culture of the sensory neurons.

Human iPSC-Derived Sensory Neuron Maximizer Kit

Human iPSC-Derived Sensory Neuron Maximizer Kit

SKU: ax0157

All in one starter kit including 1 vial of the sensory neuron progenitors, 1 bottle of Sensory Neuron Maintenance Medium, the Maturation Maximizer supplement, required growth factors, plating media and SureBond-XF coating substrate

Kit

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Phase contrast

Phase contrast images show the differentiation and maturation of axoCells Sensory Neuron Progenitors over two weeks after thawing and treatment with mitomycin C.

Phase contrast images of axoCells Sensory Neuron Progenitors The cells were plated on SureBond-XF in Neural Plating Medium. The cells were then treated with mitomycin C two days after thawing and cultured in the supplemented Sensory Neuron Maintenance Medium. (axoCells sensory neurons should be cultured for a minimum of 3 weeks prior to performing endpoint assays.)

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Sodium ion channel expression

Sodium channel RNA expression analysis by cDNA PCR

axoCells Sensory Neuron Progenitors show RNA expression of all three voltage-gated sodium ion channels, Nav1.7, Nav1.8 and Nav1.9.

cDNA from axoCells Sensory Neurons was compared to cDNA from human tissue from the dorsal root ganglion (DRG). PCR analysis (40 cycles; 55oC ) confirmed the mRNA expression of SCN9A (82 bp, hNav1.7), SCN10A (149 bp, hNav1.8) and SCN11A (464 bp, hNav1.9) in axoCells sensory neurons. SCN5a (237 bp, hNav1.5) was included as a negative control. Data provided by Dr Edward Emery (University College London).

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TTX-resistant Nav1.8 and Nav1.9

Nociceptive sensory neurons are unique in that they contain voltage-gated inward current sodium channels (Nav 1.8 and Nav 1.9) that are resistant to tetrodotoxin (TTX). The presence of these TTX-resistant ion channels was confirmed in axoCells sensory neurons.

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Electrophysiological characterization of axoCells Sensory Neurons using patch clamp. A) Phase contrast image of axoCells sensory neurons; B) Example of a sodium-current elicited by a voltage step from -100 mV to -25 mV in the presence of tetrodotoxin (0.5 mM); C) Current-voltage plot of averaged Na-currents recorded from iPSC-derived sensory neurons in the presence of TTX (n=9). Data provided by Dr Edward Emery (University College London).

Resources

Protocols & Application Notes
Posters
Product citations
Presentations
White Paper
Blogs

Protocols

User Guide: Human iPSC-derived Sensory Neuron Progenitor – How to differentiate and mature Axol sensory neuron progenitors to function neurons.

Posters

In vitro pain responses of dorsal root ganglion neurons using multi-electrode arrays
Pain responses in human iPSC-derived sensory neurons using MEA system
Electrophysiological pain responses in cultured human iPSC-derived sensory neurons using high-throughput multi-electrode array system

Presentations

Modelling Alzheimer’s Disease
Electrophysiological Maturation and Pharmacological Responses of Human induced Pluripotent Stem Cell- derived Cortical Neuronal Networks in Long-term Culture
Abnormal phosphorylation of amyloid precursor protein tyrosine residues alters the APP trafficking in neurons from Alzheimer’s Disease affected patients
Frontotemporal dementia and Parkinson’s Disease cellular models: In vitro characterization of pathological phenotypes in gene-edited iPSC-derived neurons with MAPT and LRRK2 mutations
The most sensitive microelectrode array system for in vitro extracellular electrophysiology
Pharmacological evaluation in human iPSC-derived cortical and sensory neurons using high-throughput MEA system

Application Notes

White Paper

Insights

iPSC-derived sensory neurons for the study of chemotherapy-induced neuropathic pain
Understanding the mechanisms of chemotherapy induced neuropathic pain using iPSC-derived sensory neurons
How can human iPSC-derived Sensory Neurons transform your pain research?
Expert tips on culturing human iPSC-derived Sensory Neurons to aid your research
Why make the switch from animal cells to human iPSC-derived Sensory Neurons?