An exciting scientific meeting organised by Axol Bioscience and Metrion Biosciences
We would be delighted to meet you
Make sure you save the date:
Wednesday 23 May
9:00 am – 5:00 pm
Granta Centre, Granta Park
Stem Cell Event
Hosted by Axol Bioscience & Metrion Biosciences
Axol Bioscience and Metrion Biosciences will be holding a joint stem cell themed event on May 23 rd . The meeting will consist of presentations in the morning and afternoon demonstrations in Metrion Laboratories .
Axol & Metrion stem cell event
This is a free event. Lunch and refreshments are included.
Five top tips for culturing hiPSC-Derived Sensory Neuron Progenitors
1. What types of cells will you see?
About 24 hours after thawing vials of the hiPSC-Derived Sensory Neuron Progenitors, you will see two types of cell under the microscope: fat, rounded neurons and darker, flatter cells.
Sometimes you will see more of the flat cells, because the hiPSC-Derived Sensory Neuron Progenitors are embedded in these, but don’t worry; the mitomycin C growth arrest treatment will get rid of these flat cells.
2. What happens after mitomycin C treatment?
It is normal to observe significant cell death after the mitomycin C treatment, which targets the flatter, darker, proliferating cells. This will make the population of hiPSC-Derived Sensory Neuron Progenitors more homogeneous (i.e. almost 80-90% pure sensory neurons).
From day five of cell culture, until around day ten, you’ll see the darker cells start to die and debris appear on the healthy cells, but don’t worry this all to be expected. After day 10, the sensory neurons will begin to become more prominent in the culture, with neurites becoming thicker from day 15 onwards. By this point, flat cells will be almost non-existent too.
3. How to ensure the best results from the mitomycin C treatment?
For each addition of mitomycin C to the Sensory Neuron Maintenance Medium, we advise you always make fresh mitomycin C to get the best results.
4. Don’t mistake hiPSC-Derived Sensory Neuron Progenitors for dying cells!
Under a phase contrast microscope, hiPSC-Derived Sensory Neuron Progenitors appear slightly rounder, have larger somas, and are lighter in color than other neuronal subtypes; be careful you do not mistake these for dying cells!
5. What will the sensory neurons look like after long periods in culture?
After longer culture periods (approximately five to six weeks in vitro ), neurites will become thicker and longer, and somas will become more spaced out.
The Abington Room