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Day 5: Investigating axonal biology using cerebral cortical neurons in a microfluidic culture device.

Ying Shao

Day 5: Media Change

On day 5 of our investigation into the suitability of Axol hyCCNs for studying axonal biology using microfluidic culture devices, the major step is to exchange the medium! Previously, we prepared the microfluidic device and started culturing the human cerebral cortical neurons. The expectation is that the axons will pass through the microgroove in the microfluidic device within 2 weeks of plating the cells.

• Replace the medium with fresh Complete Axol Neural Maintenance Medium.

• Check how the cortical neurons are growing on the microfluidic device.

Brightfield image of the microfluidic culture device loaded with Axol hyCCNs (ax0026F) taken at 10X magnification. The human cerebral cortical neurons were loaded in the channel on the left side of the device.  The axons are starting to appear in the right side channel of the device as they pass through the 450μm microgroove (highlighted by the arrow).

When we checked the cells under the microscope, we saw that the axons are starting to appear in the right side of the microfluidic device. How very exciting! Without wanting to tempt fate it seems like our approach is working at the first attempt!

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